Ox Bile Extract (Bovine Bile)

Bos taurus (bile)
Evidence Level
Preliminary
5 Clinical Trials
5 Documented Benefits
1/5 Evidence Score

Ox bile extract is dried bile from cattle, sold in capsules as an aid for fatty meals and marketed heavily to people who have had their gallbladder removed. Its bile acids are the body's own fat emulsifiers, so the idea is plausible, but the human evidence is three single-patient reports and one four-patient series, all in people who had lost part of the small intestine to surgery; one report used 2 g of bile acids per meal, far above a 125 to 500 mg retail capsule. No controlled trial has tested it after gallbladder removal, where chronic diarrhea often stems from too many bile acids reaching the colon, not too few. Under an FDA rule in effect since May 1991, ox bile extract is one of the digestive aid ingredients with inadequate data to be recognized as safe and effective for over-the-counter drug use; today it is sold as a dietary supplement instead.

Studied Dose One case report: 2 g conjugated bile acids from ox bile per meal; retail capsules 125-500 mg with fatty meals
Active Compound Bovine bile acids conjugated with taurine or glycine; often standardized to about 40% cholic acid

Benefits

Supports fat digestion when bile acids run short

Bile acids pack the products of fat digestion into tiny micelles that the gut lining can absorb. In published case reports of people who had lost part or all of the ileum, where bile acids are normally recycled, ox bile sharply cut the fat lost in stool, in one patient from 134 to 9 grams a day. These were single patients under medical care, not controlled trials.

Studied for nutrient absorption in short bowel syndrome

In one emaciated patient with short bowel syndrome and an ileostomy, a bile acid mixture from ox bile raised fat absorption by roughly 40 grams a day and improved calcium absorption, and over four months at 2 g per meal her weight climbed from 80 to 98 pounds. This is specialist, supervised use in a rare condition and says little about ordinary digestion.

After gallbladder removal: popular, but untested

Ox bile is marketed widely to people without a gallbladder, yet no trial has tested it in that group. The liver keeps making bile after the operation. One comparison found stool fat ran higher in men after cholecystectomy than in matched controls, but it tested no supplement. In one series, 25 of 26 people with chronic diarrhea after the surgery had bile acid malabsorption, which extra bile acids could worsen.

Bowel tolerance depends on how much bile reaches the colon

Unabsorbed bile acids that reach the colon draw in water and can cause diarrhea. In one patient with a remaining colon, stool weight did not rise on ox bile extract even as fat loss fell, but in a small Austrian series two patients with a colon had greatly worsened diarrhea on ox bile extracts. Anyone prone to loose stools should start low or skip it.

Bacterial overgrowth claims rest on rat data

Some brands suggest a bedtime capsule to keep small intestinal bacteria in check. Bile acids do have antimicrobial effects, and in rats with cirrhosis two weeks of oral conjugated bile acids returned ileal bacterial counts to normal and reduced bacterial translocation. Those rats received purified bile acids, not ox bile, and no human trial has tested ox bile for bacterial overgrowth.

Mechanism of action

1

Mixed micelles carry dietary fat

Conjugated bile acids are detergents. After lipase splits dietary fat, bile acids gather the resulting fatty acids and monoglycerides into mixed micelles that ferry them to the intestinal lining, and fat-soluble vitamins travel the same route. Too little bile acid in the upper gut lets fat, and the calories it carries, pass through unabsorbed.

2

Ileal recycling sets the size of the bile acid pool

The body reabsorbs bile acids in the terminal ileum and returns them to the liver, so each molecule is reused through several cycles before it is lost. Removing or damaging the ileum breaks that loop, the pool runs down and fat absorption fails. That is the setting of the ox bile case reports; gallbladder removal leaves the loop intact.

3

Colonic secretion when bile acids overflow

Bile acids that escape absorption and reach the colon in high concentration impair the lining and drive water secretion, producing diarrhea. This is why bile acid binders such as cholestyramine are the standard fix for bile acid diarrhea, and why supplemental bile acids can backfire in people whose colon already receives too many.

4

Natural bile acids versus a synthetic replacement

Natural conjugated bile acids, the kind in ox bile, are open to bacterial metabolism and can act as a laxative in the colon. Researchers developed cholylsarcosine, a synthetic conjugated bile acid that resists bacterial metabolism and has no cathartic activity, as a gentler replacement for people with bile acid loss.

Clinical trials

1
Ox bile in an ileectomy-ileostomy patient (case report)
PubMed

Single-patient case report of oral ox bile for severe steatorrhea (Fordtran JS, Bunch F, Davis GR 1982, Gastroenterology 82(3):564-8, PMID 7054048).

One woman with prior colectomy, partial ileectomy and ileostomy for Crohn's disease, with severe fat malabsorption from bile salt deficiency.

Her severe diarrhea improved on loperamide, but steatorrhea and malnutrition persisted until ox bile was added, which corrected both without increasing the diarrhea. The authors noted that bile salt therapy was generally avoided in such patients for fear of causing or worsening diarrhea. One patient, no control group.

2
Ox bile extract with a remaining colon (case report)
PubMed

Single-patient case report of chronic ox bile extract ingestion (Little KH, Schiller LR, Bilhartz LE, Fordtran JS 1992, Dig Dis Sci 37(6):929-33, PMID 1587199).

One patient with ileectomy and partial right colon resection for Crohn's disease and severe steatorrhea from bile acid malabsorption.

Fecal fat fell from 134 to 9 g per 24 hours, and stool weight did not increase (669 g before therapy, 507 g after). The authors concluded ox bile extract does not necessarily raise stool weight and can be tried with caution. It is a single case, so it cannot predict who will tolerate it.

3
Ox bile acids versus cholylsarcosine in short bowel syndrome
PubMed

Metabolic balance studies and a 4-month outpatient trial in one patient (Gruy-Kapral C, Little KH, Fordtran JS, Meziere TL, Hagey LR, Hofmann AF 1999, Gastroenterology 116(1):15-21, PMID 9869597).

One emaciated patient with short bowel syndrome and an ileostomy in whom parenteral nutrition could not be used.

Both a natural conjugated bile acid mixture from ox bile and synthetic cholylsarcosine increased fat absorption by about 40 g a day, calcium absorption rose, and neither caused a clinically significant rise in ileostomy output. On 2 g per meal of the natural bile acids for four months, her weight rose from 80 to 98 lb without side effects.

4
Short bowel with a remaining colon: the diarrhea tradeoff
PubMed

Uncontrolled balance studies of cholylsarcosine, with ox bile extracts compared in two patients (Kapral C, Wewalka F, Praxmarer V, Lenz K, Hofmann AF 2004, Z Gastroenterol 42(7):583-9, PMID 15248106).

Four short bowel syndrome patients with a residual colon who did not need intravenous feeding; two were also given ox bile extracts.

Cholylsarcosine raised fat absorption from 65.5 to 94.5 g a day with a 26% rise in fecal weight. In the two patients given ox bile extracts, the natural bile acids reduced steatorrhea to a smaller extent and greatly worsened diarrhea. The authors concluded the synthetic bile acid was the efficacious and safe option for patients with a colon.

5
Diarrhea after gallbladder removal: bile acid excess, not shortage
PubMed

Clinical series using the SeHCAT bile acid retention test; not a study of ox bile (Sciarretta G, Furno A, Mazzoni M, Malaguti P 1992, Am J Gastroenterol 87(12):1852-4, PMID 1449156).

33 patients after cholecystectomy, 26 of them with chronic diarrhea.

Marked bile acid malabsorption was found in 25 of the 26 patients with diarrhea, and the bile acid binder cholestyramine at 2 to 12 g a day was effective in 23 of 25. Adding bile acids runs opposite to the treatment that worked here, and ox bile has never been tested in this group.

Side effects and drug interactions

Common Potential side effects

Loose stools, diarrhea and cramping, especially at higher doses or in people who still have a colon.
Stomach upset or other digestive distress; at least one retail label advises stopping or lowering the dose if this occurs.
May aggravate diarrhea after gallbladder removal, which is often driven by bile acid malabsorption.
Not for use with a blocked bile duct, active gallbladder or bile duct disease, or unexplained jaundice without medical advice.
Derived from cattle, so unsuitable for vegetarians, vegans and anyone avoiding bovine products.
Long-term safety at supplement doses, and safety in pregnancy and breastfeeding, have not been studied.

Important Drug interactions

Cholestyramine, colestipol and colesevelam: these bile acid binders soak up bile acids and would cancel out ox bile; they are also the usual treatment for bile acid diarrhea, the opposite aim.
Ursodiol and prescription cholic acid: ox bile adds to a prescribed bile acid load; clear it with the prescriber first.
Cyclosporine: oral cyclosporine absorption has been shown to rise when more bile reaches the gut, so transplant patients should not add ox bile without their care team.
Orlistat: it blocks fat absorption on purpose, so pairing it with a fat-absorption aid works at cross purposes.
Laxatives and other drugs that loosen stools: bile acids reaching the colon speed transit and may add to the effect.

Frequently asked questions about Ox Bile Extract (Bovine Bile)

What is Ox Bile Extract?

Ox bile extract is dried bile from cattle, sold in capsules as an aid for fatty meals and marketed heavily to people who have had their gallbladder removed. Its bile acids are the body's own fat emulsifiers, so the idea is plausible, but the human evidence is three single-patient reports and one four-patient series, al…

What is Ox Bile Extract used for?

Ox Bile Extract is researched primarily for Gut Health. Bile acids pack the products of fat digestion into tiny micelles that the gut lining can absorb. In published case reports of people who had lost part or all of the ileum, where bile acids are normally recycled, ox bile sharply cut the fat…

What is the recommended dosage of Ox Bile Extract?

The clinically studied dose is One case report: 2 g conjugated bile acids from ox bile per meal; retail capsules 125-500 mg with fatty meals Always follow the product label and check with a healthcare provider for personal advice.

Is Ox Bile Extract safe, and does it have side effects?

For most healthy adults, Ox Bile Extract is well tolerated at studied doses. Reported effects can include: Loose stools, diarrhea and cramping, especially at higher doses or in people who still have a colon. Stomach upset or other digestive distress; at least one retail label advises stopping or lowering the dose if this occurs. It may also interact with some medications. Ox Bile Extract is not right for everyone, so check with a healthcare provider first if you are pregnant or breastfeeding, have a medical condition, or take prescription medication.

Does Ox Bile Extract interact with any medications?

Possible interactions include: Cholestyramine, colestipol and colesevelam: these bile acid binders soak up bile acids and would cancel out ox bile; they are also the usual treatment for bile acid diarrhea, the opposite aim. If you take prescription medication, check with a pharmacist or doctor before using it.

How strong is the scientific evidence for Ox Bile Extract?

NutraSmarts rates the evidence for Ox Bile Extract as Preliminary (1 out of 5). It is backed by 5 clinical trials and 8 cited references summarized on this page. A higher rating reflects more, larger, and better-designed human studies.

References(8 citations)

Evidence ratings on NutraSmarts are based on the totality of human clinical research, with emphasis on randomized controlled trials, meta-analyses, and systematic reviews. The references below directly support claims made throughout this page.

  1. Fordtran JS, Bunch F, Davis GR. Ox bile treatment of severe steatorrhea in an ileectomy-ileostomy patient. Gastroenterology. 1982;82(3):564-8..PubMedUsed to support: Single-patient case report in which ox bile corrected severe steatorrhea and malnutrition without increasing diarrhea in a woman with colectomy, partial ileectomy and ileostomy for Crohn's disease. It also documents why bile salt therapy was usually avoided: fear of worsening diarrhea.
  2. Little KH, Schiller LR, Bilhartz LE, Fordtran JS. Treatment of severe steatorrhea with ox bile in an ileectomy patient with residual colon. Dig Dis Sci. 1992;37(6):929-33..PubMedUsed to support: Single-patient case report: chronic ox bile extract cut fecal fat from 134 to 9 g per 24 hours while stool weight went from 669 to 507 g, in a patient who still had a colon after partial right colon resection. The authors advised it can be tried with caution.
  3. Gruy-Kapral C, Little KH, Fordtran JS, Meziere TL, Hagey LR, Hofmann AF. Conjugated bile acid replacement therapy for short-bowel syndrome. Gastroenterology. 1999;116(1):15-21..PubMedUsed to support: One patient with short bowel syndrome and an ileostomy: a natural conjugated bile acid mixture from ox bile and synthetic cholylsarcosine each raised fat absorption by about 40 g a day and improved calcium absorption; 2 g per meal of natural bile acids for four months took her weight from 80 to 98 lb.
  4. Kapral C, Wewalka F, Praxmarer V, Lenz K, Hofmann AF. Conjugated bile acid replacement therapy in short bowel syndrome patients with a residual colon. Z Gastroenterol. 2004;42(7):583-9..PubMedUsed to support: The main contrary evidence on this page: in two short bowel patients with a residual colon, ox bile extracts reduced steatorrhea less than cholylsarcosine and greatly worsened diarrhea. An uncontrolled study of four patients.
  5. Sciarretta G, Furno A, Mazzoni M, Malaguti P. Post-cholecystectomy diarrhea: evidence of bile acid malabsorption assessed by SeHCAT test. Am J Gastroenterol. 1992;87(12):1852-4..PubMedUsed to support: Among 26 cholecystectomy patients with chronic diarrhea, 25 had marked bile acid malabsorption and most responded to the bile acid binder cholestyramine. Cited for the point that diarrhea after gallbladder removal usually reflects excess bile acids in the colon; it did not test ox bile.
  6. Brydon WG, Ross AH, Anderson JR, Douglas S. Diet and faecal lipids following cholecystectomy in men. Digestion. 1982;25(4):248-52..PubMedUsed to support: Men after cholecystectomy excreted more fecal fat and had heavier stools than age-matched controls on similar diets, while bile acid excretion did not differ significantly. The only fat-digestion data on this page for people without a gallbladder; no supplement was tested.
  7. Hofmann AF. The continuing importance of bile acids in liver and intestinal disease. Arch Intern Med. 1999;159(22):2647-58..PubMedUsed to support: Background physiology for the mechanism section: bile acids are conjugated with glycine or taurine, recycle through multiple enterohepatic circulations, solubilize dietary lipids for absorption, and impair colonic mucosal function at high colonic concentrations, where bile acid sequestrants relieve diarrhea.
  8. Lorenzo-Zúñiga V, Bartolí R, Planas R, Hofmann AF, Viñado B, Hagey LR, Hernández JM, Mañé J, Alvarez MA, Ausina V, Gassull MA. Oral bile acids reduce bacterial overgrowth, bacterial translocation, and endotoxemia in cirrhotic rats. Hepatology. 2003;37(3):551-7..PubMedUsed to support: Animal study only: in rats with cirrhosis and ascites, two weeks of oral cholylsarcosine or cholylglycine normalized ileal bacterial content and reduced bacterial translocation and endotoxemia. It used purified bile acids, not ox bile, and is the only support for the small intestinal bacteria claim on this page.