Lactobacillus johnsonii La1 (NCC533 / CNCM I-1225)

Lactobacillus johnsonii La1 = NCC533 = CNCM I-1225 (Nestlé strain)
Evidence Level
Limited
3 Clinical Trials
6 Documented Benefits
2/5 Evidence Score

A specific Nestlé probiotic strain, also sold under the names NCC533 and CNCM I-1225. Almost all of the human research on it asks one narrow question: whether it lowers the amount of Helicobacter pylori bacteria living in the stomach. Originally classified as Lactobacillus acidophilus group A3, later reorganized to L. johnsonii. There are three small human studies. The main one, published in 1999, gave 20 adults a cell-free whey liquid made from La1 cultures rather than the live bacteria, with one group also taking the prescription acid blocker omeprazole 20 mg four times a day; stomach biopsies confirmed the bacteria were reduced but still there. A 2003 study in symptom-free volunteers found a similar partial reduction, and a 2008 trial tested cranberry juice and La1 in children aged 6 to 16 who carried the bacteria. The gastritis and anti-inflammatory findings come from mice, not people. Nestlé holds a patent on the strain, but a patent is a legal filing and is not evidence that something works. Every effect reported is partial suppression, never clearance. H. pylori is a diagnosed infection linked to ulcers and stomach cancer; it needs medical eradication treatment, and this strain is not a treatment for it. No cited study measured any immune outcome.

Studied Dose The 1999 study tested a cell-free liquid made from La1 cultures, described as roughly 10^9 CFU per mL of starting culture, which itself contains no live bacteria. Other work used fermented milk or yogurt at roughly 10^10 CFU per day. Doses were not consistent across the three human studies.
Active Compound Lactobacillus johnsonii La1 = NCC533 = CNCM I-1225 (Nestle strain); reclassified from L. acidophilus group A3.

Benefits

Cell-free La1 liquid partly lowered H. pylori in 20 adults

In a randomized double-blind study of 20 adults with an average age of 33 (PMID 10343133), a cell-free whey liquid made from La1 cultures, not the live bacteria, produced a partial, long-term drop in H. pylori that did not depend on changing stomach acid. One group also took prescription omeprazole 20 mg four times a day, and follow-up stomach biopsies confirmed the infection was still present. This is a small early study in people who already had an infection, so it says nothing about what La1 does for a healthy person.

Partial drop in H. pylori carriage in symptom-free volunteers

In a 2003 study of volunteers who carried H. pylori without symptoms (PMID 12697639), taking La1 often lowered how much of the bacteria was present. The drop was partial and the bacteria were not cleared. Everyone in the study was symptom-free, so the result says nothing about easing stomach complaints.

Tested in children aged 6 to 16 who carried H. pylori

The only reference cited on this page (PMID 18343637, Nutrition 2008) is a randomized multicentre trial in children aged 6 to 16 who were carrying H. pylori without symptoms. It tested cranberry juice, La1, or both, and the authors concluded only that regular intake of either may be useful in managing symptom-free colonization in children. It is a paediatric infection study, not evidence of a general gut benefit in adults.

Reduced stomach inflammation in mice (not people)

In C57BL/6 mice, La1 eased H. pylori-related stomach inflammation and lowered inflammatory signalling proteins. This is animal work only. No human study on this page measured gastritis or inflammation, so this finding should not be read as something La1 does in people.

Lactacin F: a lab-bench antimicrobial mechanism

L. johnsonii makes lactacin F, a bacteriocin that punches holes in the membranes of other bacteria in laboratory tests. This is a test-tube observation about how the strain might act. No study cited here shows that lactacin F produces any measurable benefit in people.

Suppressive vs eradicative — honest framing

In the studies, La1 partly lowered the amount of H. pylori over time but never cleared it. Does not replace standard triple/quadruple H. pylori eradication therapy. H. pylori infection is diagnosed and treated by a doctor, and nothing here is a substitute for that care.

Mechanism of action

1

Lactacin F bacteriocin pore formation

In laboratory tests, lactacin F forms pores in the membranes of other bacteria and disrupts them. This has been shown on the bench, not in people.

2

Whey-based culture supernatant activity

The 1999 human study used the liquid left after growing La1, filtered free of the live bacteria, which means the effect seen there cannot be credited to a live probiotic. Products sold as live La1 capsules or fermented milk are not the same thing that was tested in that study.

3

Mucus thickness enhancement

La1 has been reported to thicken the stomach's mucus layer, which would put more distance between H. pylori and the stomach lining. This comes from laboratory and animal work, not from any of the human studies cited here.

4

Adhesin competition with H. pylori

In laboratory work, La1 can compete for the same asialo-GM1 and sulfatide binding sites on stomach cells that H. pylori uses to attach. Whether this competition happens meaningfully in a living human stomach has not been shown.

5

Acid-independent suppression

The 1999 study reported that the suppression did not appear to depend on stomach acid, since it was seen with and without the acid-blocking drug omeprazole. That is one observation from a 20-person study, not an established mechanism.

6

Proinflammatory chemokine reduction

In mice, La1 lowered inflammatory signalling proteins in the stomach. Mouse immune findings do not transfer to people, and no human study cited here measured inflammation or any immune outcome.

Clinical trials

1
Cell-Free La1 Whey Liquid, 20 Adults With H. pylori

Michetti P, Dorta G, Wiesel PH et al.

Clinical population described in trial publication.

Michetti P, Dorta G, Wiesel PH and colleagues, Digestion 1999;60(3):203-209 (PMID 10343133). Randomized, double-blind study in 20 adults (8 women, 12 men, average age 33) infected with H. pylori. What was tested was a cell-free whey liquid made from La1 cultures, not live probiotic bacteria, and one group also received prescription omeprazole 20 mg four times daily. The authors reported a partial, acid-independent, long-term suppressive effect, and stomach biopsies confirmed the infection was still present. Twenty people is a very small study and the bacteria were never eliminated.

2
La1 in Symptom-Free H. pylori Carriers

Frequent ingestion of La1 in asymptomatic H. pylori-positive volunteers showed suppressive effect on colonization.

Clinical population described in trial publication.

Gotteland M and Cruchet S, Journal of Antimicrobial Chemotherapy 2003;51(5):1317-1319 (PMID 12697639). Frequent intake of La1 lowered H. pylori colonization in volunteers who carried the bacteria without symptoms. The effect was a reduction in bacterial numbers only. No symptom, inflammation or immune outcomes were measured, and the bacteria were not eradicated.

3
Mouse Study: La1 and H. pylori Stomach Inflammation

Animal study in mice. Not a clinical trial and not evidence of an effect in people.

Clinical population described in trial publication.

C57BL/6 mouse model. La1 eased H. pylori-related stomach inflammation and lowered inflammatory signalling proteins. This is preclinical animal work, included for background only. No human study on this page measured gastritis or inflammation.

Side effects and drug interactions

Common Potential side effects

Generally well-tolerated; food-grade probiotic origin (Nestlé fermented milk products).
Mild GI upset (rare; transient).
Pregnancy and breastfeeding: not specifically studied. Ask your doctor before using.
Long-term safety: the strain has a long history of use in fermented dairy foods, but only three small human studies have been published, so long-term data are limited.
Allergic reactions in milk-derived ingredient sensitivities (rare).
Severely immunocompromised individuals: caution (applies to all probiotics).
The strain is a Nestlé product and the company has a commercial interest in the research on it, which is worth keeping in mind when reading the results.

Important Drug interactions

Antibiotics: La1 has not been shown to add anything to standard H. pylori antibiotic treatment. If you have a diagnosed infection, follow the eradication therapy your doctor prescribes.
Most medications: no interactions have been reported, though only three small studies exist.
Immunosuppressants: caution (applies to all probiotics).
Other probiotics: compatible.
Anticoagulants: no interactions documented.
Acid-blocking drugs: in the 1999 study one group took omeprazole 20 mg four times daily alongside the La1 preparation, with no problems reported.

Frequently asked questions about Lactobacillus johnsonii La1 (NCC533 / CNCM I-1225)

What is Lactobacillus johnsonii La1 used for?

La1 (also called NCC533) has been studied almost entirely for one thing: whether it lowers the amount of Helicobacter pylori bacteria in the stomach. Three small human studies found partial reductions, but the bacteria were never cleared. No cited study measured immune function or the gut lining, and La1 is not a treatment for H. pylori infection, which needs medical care.

How much L. johnsonii La1 should I take?

It is dosed in the billions of CFU per day, often delivered in fermented dairy or capsules; follow the specific product's labeling. Strain identity matters, so check the label says La1. Note that the main 1999 study tested a cell-free liquid rather than live bacteria, so no supplement on the market matches exactly what was studied.

When should I take L. johnsonii La1?

Once daily, with or before a meal. For gut and stomach-related goals, consistent use over several weeks is the studied approach.

Is L. johnsonii La1 safe?

It is generally very safe and well tolerated. Severely immunocompromised or critically ill people should check with a doctor before taking live probiotics.

What is Lactobacillus johnsonii La1?

A specific Nestlé probiotic strain, also sold under the names NCC533 and CNCM I-1225. Almost all of the human research on it asks one narrow question: whether it lowers the amount of Helicobacter pylori bacteria living in the stomach. Originally classified as Lactobacillus acidophilus group A3, later reorganized to L.

What is the recommended dosage of Lactobacillus johnsonii La1?

The clinically studied dose is The 1999 study tested a cell-free liquid made from La1 cultures, described as roughly 10^9 CFU per mL of starting culture, which itself contains no live bacteria. Other work used fermented milk or yogurt at roughly 10^10 CFU per day. Always follow the product label and check with a healthcare provider for personal advice.

Is Lactobacillus johnsonii La1 safe, and does it have side effects?

For most healthy adults, Lactobacillus johnsonii La1 is well tolerated at studied doses. Reported effects can include: Generally well-tolerated; food-grade probiotic origin (Nestlé fermented milk products). Mild GI upset (rare; transient). It may also interact with some medications. Lactobacillus johnsonii La1 is not right for everyone, so check with a healthcare provider first if you are pregnant or breastfeeding, have a medical condition, or take prescription medication.

Does Lactobacillus johnsonii La1 interact with any medications?

Possible interactions include: Antibiotics: La1 has not been shown to add anything to standard H. pylori antibiotic treatment. If you have a diagnosed infection, follow the eradication therapy your doctor prescribes. Most medications: no interactions have been reported, though only three small studies exist. If you take prescription medication, check with a pharmacist or doctor before using it.

How strong is the scientific evidence for Lactobacillus johnsonii La1?

NutraSmarts rates the evidence for Lactobacillus johnsonii La1 as Limited (2 out of 5). It is backed by 3 clinical trials and 3 cited references summarized on this page. A higher rating reflects more, larger, and better-designed human studies.

References(3 citations)

Evidence ratings on NutraSmarts are based on the totality of human clinical research, with emphasis on randomized controlled trials, meta-analyses, and systematic reviews. The references below directly support claims made throughout this page.

  1. Gotteland M, Andrews M, Toledo M, et al. Modulation of Helicobacter pylori colonization with cranberry juice and Lactobacillus johnsonii La1 in children. Nutrition. 2008;24(5):421-6..PubMedUsed to support: Randomized multicentre trial in children aged 6 to 16 who were carrying H. pylori without symptoms, testing cranberry juice, La1, or both. The authors concluded that regular intake of either may be useful in managing symptom-free colonization in children.
  2. Michetti P, Dorta G, Wiesel PH, Brassart D, Verdu E, Herranz M, Felley C, Porta N, Rouvet M, Blum AL, Corthésy-Theulaz I. Effect of whey-based culture supernatant of Lactobacillus acidophilus (johnsonii) La1 on Helicobacter pylori infection in humans. Digestion. 1999;60(3):203-209..PubMedUsed to support: Randomized double-blind trial, n=20, of a CELL-FREE whey-based La1 culture supernatant (not live bacteria) given with omeprazole or placebo for 14 days. Authors: 'La1 culture supernatant ... has a partial, acid-independent long-term suppressive effect on H. pylori in humans.' Persistent infection was still confirmed on gastric biopsy, so this is suppression, not eradication.
  3. Gotteland M, Cruchet S. Suppressive effect of frequent ingestion of Lactobacillus johnsonii La1 on Helicobacter pylori colonization in asymptomatic volunteers. Journal of Antimicrobial Chemotherapy. 2003;51(5):1317-1319..PubMedUsed to support: Frequent ingestion of live La1 reduced H. pylori colonization in asymptomatic volunteers. A suppressive effect on colonization, not eradication of the infection.