Evidence Level
Limited
3 Clinical Trials
5 Documented Benefits
2/5 Evidence Score

Kaempferol is a flavonoid antioxidant found widely in plant foods such as kale, broccoli, beans, tea, and berries, and is one reason a vegetable-rich diet is associated with health benefits. Its antioxidant and anti-inflammatory activity has been shown in laboratory work, and people who eat more kaempferol-rich food have modestly lower cardiovascular risk in population studies. The only studies of isolated kaempferol in people are five small studies, all run by a single company, Otsuka Pharmaceutical Co., Ltd., that used 10 mg, or 50 mg in one safety study, and measured exercise oxygen cost, heart rate, physical activity, sleep and tolerability. The one trial that did check C-reactive protein, blood pressure, cholesterol, glucose and HbA1c was a 4-week safety study at 50 mg/day, and none of those measures improved. No human study of kaempferol has measured an oxidative-stress marker at all. Isolated kaempferol supplements are uncommon and doses are not well standardized, so food sources remain the simplest way to obtain it. Dietary kaempferol is very safe, and supplements are generally well tolerated, though flavonoids can interact with some medications.

Studied Dose Every human trial of isolated kaempferol used 10 mg, the highest ever 50 mg/day; commercial 50 to 200 mg capsules have never been tested, and there is no established clinical dose.
Active Compound Kaempferol (3,5,7-trihydroxy-2-(4-hydroxyphenyl)-4H-1-benzopyran-4-one) and its glycosides.

Benefits

Cardiovascular Risk Reduction (Epidemiological)

A dose-response meta-analysis showed kaempferol intake is linearly associated with lower risk of cardiovascular disease, and quercetin showed a similar inverse association with coronary heart disease. Cohort evidence is not consistent, however. In the Nurses' Health Study (66,360 women, 12 years, 938 non-fatal myocardial infarctions and 324 coronary deaths) there was no association between flavonol or flavone intake and non-fatal myocardial infarction or fatal coronary heart disease; the only signal was a weak reduction in coronary death in the highest kaempferol quintile (relative risk 0.66, 95% CI 0.48-0.93, p for trend 0.04), which the authors attributed largely to broccoli consumption rather than to kaempferol itself. All of this measures kaempferol eaten in food, not kaempferol taken as a supplement. The only randomised trial to measure cardiovascular numbers directly was a 4-week safety study of 50 mg/day in 48 healthy adults, in which blood pressure, total, HDL and LDL cholesterol and triglycerides did not improve (Akiyama 2023, PMID 37701215). No trial has measured endothelial function or any cardiovascular event.

Growth-Signaling Effects in Cultured Cells

In cultured cell lines kaempferol alters several growth-signaling pathways, including PI3K/AKT, and changes the expression of adhesion and matrix proteins such as matrix metallopeptidase 2. These experiments use micromolar concentrations; the mean peak plasma concentration in people after a 9 mg oral dose is about 0.1 micromolar (DuPont 2004, PMID 15164116), so the laboratory concentrations are roughly 100 to 1,000 times higher than anything oral kaempferol produces in the body. Nothing here has been shown to happen in a person. Kaempferol is not a treatment for any disease. The population data do not support a preventive role either: in the Women's Health Study (38,408 women, 11.5 years, 3,234 incident cancers) intake of kaempferol and the other quantified flavonols showed no association with total cancer or with breast, colorectal, lung, endometrial or ovarian cancer, and the authors concluded their results do not support a major role for these compounds in cancer prevention.

Anti-inflammatory Activity

Kaempferol inhibits NF-κB and AP-1 signaling, MAPK pathways, and pro-inflammatory cytokine production in vitro. Reduces iNOS and COX-2 expression. All of this is cell-culture work at concentrations well above what oral kaempferol reaches in human plasma. The only human trial to measure an inflammatory marker was a 4-week safety study of 50 mg/day in 48 healthy adults, in which C-reactive protein did not fall (Akiyama 2023, PMID 37701215); no trial has measured IL-6, TNF-alpha or any other inflammatory marker. An anti-inflammatory effect in people has not been demonstrated.

Glucose Metabolism in Animal Models

Animal studies show kaempferol suppresses IKK/NF-κB hepatic signaling, IRS-1 phosphorylation, and significantly enhances insulin secretion and synthesis. Improves insulin sensitivity in rodent models. No human trial has tested kaempferol for blood-sugar control. The only trial to measure glucose and HbA1c at all was a 4-week safety study of 50 mg/day in healthy adults, in which neither improved (Akiyama 2023, PMID 37701215); insulin was not measured. None of the rodent findings has been shown to occur in people, and kaempferol should not be used in place of diabetes care.

Antioxidant Activity

Like other flavonols, kaempferol scavenges free radicals and induces endogenous antioxidant systems via Nrf2 activation. Antioxidant capacity is comparable to other major flavonoids (quercetin, myricetin) in chemical assays. Those are test-tube measurements. No randomised or clinical trial of kaempferol has reported oxidative-stress markers such as malondialdehyde or F2-isoprostanes in people, so antioxidant activity inside the human body has not been demonstrated.

Mechanism of action

1

NF-κB and AP-1 Pathway Inhibition

Kaempferol inhibits IκB kinase (IKK), preventing NF-κB activation. Also suppresses AP-1 transcription factor activity. In cell culture this reduces expression of inflammatory cytokines and proliferation-related genes. It is the proposed explanation for kaempferol's laboratory anti-inflammatory activity. Whether it occurs at all at the concentrations oral kaempferol reaches in human tissue is unknown.

2

PI3K/AKT/mTOR Pathway Modulation

In cancer cells, kaempferol downregulates PI3K/AKT signaling — reducing pro-survival signaling and inducing apoptosis. The same pathway is speculatively linked to insulin signalling, but this has not been shown in humans.

3

Programmed Cell Death in Cultured Cell Lines

In cell-culture experiments kaempferol triggers apoptosis in immortalised cell lines, with relative sparing of normal cells in some studies. These experiments use micromolar concentrations that oral kaempferol does not reach in human blood - peak plasma is about 0.1 micromolar after a 9 mg dose (DuPont 2004, PMID 15164116). This is laboratory pharmacology only. Kaempferol is not a cancer treatment and has never been tested as one in people.

4

Cell-Adhesion and Migration Markers in Cell Culture

In cultured cells kaempferol changes expression of epithelial-mesenchymal transition markers (N-cadherin, E-cadherin, Slug, Snail) and of matrix metallopeptidase 2. This is descriptive cell biology at concentrations not achievable from oral intake, and it has no demonstrated consequence in humans.

5

Free Radical Scavenging and Nrf2 Activation

Direct ROS scavenging attributed mainly to the 3-hydroxyl group and the 4'-hydroxyl on the B ring. Indirect antioxidant effects via Nrf2-mediated induction of phase II detoxification enzymes (HO-1, NQO1, glutathione synthesis). These are proposed antioxidant mechanisms demonstrated in cells and animals. No human study has confirmed that oral kaempferol activates Nrf2 or changes any oxidative-stress marker in people.

Clinical trials

1
Okita 2025 — Single 10 mg Kaempferol Dose and Exercise Oxygen Cost (Randomised Crossover)

Randomised, double-blind, placebo-controlled crossover trial of a single 10 mg oral dose of kaempferol, with constant-load exercise tests at 25%, 50%, 75%, 100% and 125% of VO2max. (Okita K, Mizokami T, Yasuda O, Ikeda Y. Physiol Rep. 2025;13(9):e70369. PMID 40346019. Registered UMIN000049589. Two of the four authors are affiliated with Otsuka Pharmaceutical Co., Ltd.; the paper itself declares no conflict of interest.)

16 well-trained male university athletes (mean VO2max 57.5 mL/kg/min).

Kaempferol significantly reduced oxygen uptake and respiratory rate during submaximal exercise at 25%, 50% and 75% of VO2max compared with placebo, without raising the respiratory quotient or blood lactate, and significantly extended exercise duration at 100% of VO2max. Limits: one small acute-dose crossover study in trained young men, run by the company that sells the ingredient, with no independent replication; an erratum has since been published (Physiol Rep 2025;13(16):e70518). It measured no antioxidant, inflammatory or cardiovascular-risk endpoint.

2
Akiyama 2023 — 50 mg/day Kaempferol Aglycone for 4 Weeks (Safety RCT)

Randomised, double-blind, placebo-controlled 4-week trial of 50 mg/day kaempferol aglycone capsules, roughly five times estimated dietary intake, against a cornstarch placebo. (Akiyama M, Mizokami T, Ito H, Ikeda Y. Food Sci Nutr. 2023;11(9):5427-5437. PMID 37701215. All four authors employed by Otsuka Pharmaceutical Co., Ltd.)

48 healthy adults, 24 receiving kaempferol and 24 placebo, for 4 weeks.

The safety panel covered blood pressure, body composition, haematology, liver and kidney chemistry, C-reactive protein, total, HDL and LDL cholesterol, triglycerides, glucose and HbA1c, plus urinalysis. Nothing improved relative to placebo, and no adverse events were attributable to kaempferol. That makes this card doubly important: it establishes short-term tolerability at 50 mg/day, and it is also the only human data in existence on kaempferol and CRP, blood pressure, cholesterol or blood sugar - none of which moved. It measured no oxidative-stress marker. It is also the highest dose of isolated kaempferol ever given to people in a trial, which is why capsules sold at 100-200 mg have no human safety data behind them.

3
Ikeda 2024 — 10 mg/day Kaempferol for 2 Weeks: Heart Rate, Activity and Sleep (Randomised Crossover)

Double-blind, placebo-controlled, randomised crossover trial of 10 mg/day kaempferol for 2 weeks with a 7-day washout, with activity, heart rate and heart-rate variability recorded continuously by wrist accelerometer. (Ikeda Y, Gotoh-Katoh A, Okada S, Handa S, Sato T, Mizokami T, Saito B. Front Nutr. 2024;11:1386389. PMID 39155930. Registered UMIN000042438. Three authors employed by Otsuka Pharmaceutical Co., Ltd.)

33 city workers (17 men, 16 women).

Heart rate fell at every activity level, mean daily step count and distance covered rose, and self-reported sleep quality improved during kaempferol compared with placebo. Limits: a small crossover trial run by a company with a commercial interest in kaempferol, with device-recorded activity and self-report as the outcomes. No blood pressure, lipid, inflammatory or oxidative-stress endpoint was measured, and the results have not been independently replicated.

Side effects and drug interactions

Common Potential side effects

Generally well-tolerated as a dietary constituent — kaempferol is consumed in substantial amounts via vegetables, tea, and fruits.
Possible mild GI symptoms at high supplemental doses. Note that the highest dose ever tested in a human trial is 50 mg/day for 4 weeks (Akiyama 2023, PMID 37701215), which produced no adverse events; above that, tolerability is simply unknown rather than established as safe.
Kaempferol is one of the more potent dietary inhibitors of thyroid peroxidase, the enzyme that makes thyroid hormone. In a structure-activity study of 13 commonly consumed flavonoids, inhibition IC50 values ranged from 0.6 to 41 micromolar, and kaempferol was among the most potent, acting by mechanism-based (irreversible) inactivation, which is longer-lasting than reversible inhibition (Divi and Doerge 1996, PMID 8924586). This is enzyme chemistry rather than a demonstrated effect in people, and peak plasma kaempferol after a normal oral dose is around 0.1 micromolar, well below the inhibitory range - but it is a real reason for caution with concentrated high-dose supplements, particularly for anyone with existing thyroid disease or low iodine intake, rather than with kaempferol from food.
Possible mild antiplatelet effect — relevant for those on anticoagulants.
Pregnancy: dietary amounts are safe (consumed in foods); concentrated supplemental kaempferol lacks specific pregnancy safety data — exercise caution.
Lactation: likely safe in dietary amounts.
Drug-metabolizing enzyme effects (CYP modulation) at high doses — theoretical drug interaction risk.

Important Drug interactions

Anticoagulants/antiplatelets (warfarin, aspirin, clopidogrel): theoretical mild antiplatelet effects — monitor.
Cyclosporine, tacrolimus, statins: theoretical CYP3A4 modulation at high doses.
Diabetes medications: theoretical mild blood-glucose effects.
Quercetin and other flavonol supplements: similar mechanisms — additive effects rather than concerning interactions.
Aluminum-containing antacids: kaempferol may chelate metal ions — separate doses by 2 hours.

Frequently asked questions about Kaempferol

What is kaempferol used for?

Kaempferol is a flavonoid found in kale, broccoli, tea, and many fruits and vegetables. Antioxidant and anti-inflammatory activity has been shown in laboratory studies, and people who eat more kaempferol-rich food have modestly lower cardiovascular risk in population studies. The few trials of kaempferol supplements in people mostly measured exercise oxygen use, heart rate, physical activity and sleep quality. The one trial that did check C-reactive protein, blood pressure, cholesterol and blood sugar was a 4-week safety study, and none of them improved.

What foods contain kaempferol?

It is widespread in plant foods, including leafy greens, broccoli, beans, tea, and berries, which is one reason a vegetable-rich diet is associated with health benefits. Supplements concentrate it, but food sources are abundant.

How much kaempferol should I take?

There is no established dose. Human trials have used 10 mg a day, and a single 4-week safety study used 50 mg a day. Capsules sold at 100-200 mg have not been tested in people at all, so 'follow the label' means following a number the manufacturer chose rather than one a study supports. Most of the research reflects kaempferol eaten in plant foods rather than concentrated supplements.

Is kaempferol safe?

As a flavonoid abundant in foods, dietary kaempferol is very safe. Concentrated supplements are generally well tolerated, but long-term high-dose data is limited, and flavonoids can interact with some medications, so check with your doctor.

What is Kaempferol?

Kaempferol is a flavonoid antioxidant found widely in plant foods such as kale, broccoli, beans, tea, and berries, and is one reason a vegetable-rich diet is associated with health benefits.

What is the recommended dosage of Kaempferol?

The clinically studied dose is Every human trial of isolated kaempferol used 10 mg, the highest ever 50 mg/day; commercial 50 to 200 mg capsules have never been tested, and there is no established clinical dose. Always follow the product label and check with a healthcare provider for personal advice.

Is Kaempferol safe, and does it have side effects?

For most healthy adults, Kaempferol is well tolerated at studied doses. Reported effects can include: Generally well-tolerated as a dietary constituent — kaempferol is consumed in substantial amounts via vegetables, tea, and fruits. Possible mild GI symptoms at high supplemental doses. It may also interact with some medications. Kaempferol is not right for everyone, so check with a healthcare provider first if you are pregnant or breastfeeding, have a medical condition, or take prescription medication.

Does Kaempferol interact with any medications?

Possible interactions include: Anticoagulants/antiplatelets (warfarin, aspirin, clopidogrel): theoretical mild antiplatelet effects — monitor. Cyclosporine, tacrolimus, statins: theoretical CYP3A4 modulation at high doses. If you take prescription medication, check with a pharmacist or doctor before using it.

How strong is the scientific evidence for Kaempferol?

NutraSmarts rates the evidence for Kaempferol as Limited (2 out of 5). It is backed by 3 clinical trials and 14 cited references summarized on this page. A higher rating reflects more, larger, and better-designed human studies.

References(14 citations)

Evidence ratings on NutraSmarts are based on the totality of human clinical research, with emphasis on randomized controlled trials, meta-analyses, and systematic reviews. The references below directly support claims made throughout this page.

  1. Micek A, Godos J, Del Rio D, Galvano F, Grosso G Dietary Flavonoids and Cardiovascular Disease: A Comprehensive Dose-Response Meta-Analysis Molecular Nutrition & Food Research. 2021;65(6):e2001019. doi:10.1002/mnfr.202001019.PubMedUsed to support: The strongest evidence on this page, and it is dietary. Dose-response meta-analysis of 39 prospective cohort studies (1,501,645 individuals; 33,637 CVD, 23,664 CHD and 11,860 stroke cases) in which KAEMPFEROL WAS ANALYSED AS AN INDIVIDUAL COMPOUND, not merely as part of the flavonol class: increasing kaempferol intake was linearly associated with lower risk of cardiovascular disease (and quercetin intake with lower risk of coronary heart disease). LIMITS: observational cohorts only, measuring kaempferol consumed in whole foods via food-frequency questionnaires. Confounding by overall vegetable and fruit intake cannot be separated out, and the finding does not transfer automatically to an isolated kaempferol capsule, which no trial has tested against any cardiovascular event or endothelial endpoint.
  2. Lin J, Rexrode KM, Hu F, Albert CM, Chae CU, Rimm EB, Stampfer MJ, Manson JE Dietary intakes of flavonols and flavones and coronary heart disease in US women American Journal of Epidemiology. 2007;165(11):1305-13. doi:10.1093/aje/kwm016.PubMedUsed to support: MISATTRIBUTED AS CURRENTLY WRITTEN; the primary result was null. Prospective cohort (Nurses' Health Study, 66,360 women, 12 years of follow-up 1990-2002, 938 non-fatal myocardial infarctions and 324 coronary deaths). The authors observed NO association between flavonol or flavone intake and risk of non-fatal myocardial infarction or fatal coronary heart disease. The single positive signal was a weak reduction in CHD DEATH ONLY among women in the highest kaempferol quintile (multivariate RR 0.66, 95% CI 0.48-0.93, p for trend 0.04), which the authors state was probably attributable to broccoli consumption. Their stated conclusion is that these prospective data do not support an inverse association between flavonol or flavone intake and CHD risk. This is dietary intake from food, not supplementation.
  3. Wang L, Lee IM, Zhang SM, Blumberg JB, Buring JE, Sesso HD Dietary intake of selected flavonols, flavones, and flavonoid-rich foods and risk of cancer in middle-aged and older women The American Journal of Clinical Nutrition. 2009;89(3):905-12. doi:10.3945/ajcn.2008.26913.PubMedUsed to support: NULL RESULT, cited here for honesty rather than support. Prospective cohort (Women's Health Study, 38,408 women aged 45 and over, 11.5 years of follow-up, 3,234 incident cancers) in which kaempferol was quantified as an individual flavonol. Intake of total quantified flavonoids showed no association with total cancer (multivariate RRs across quintiles 1.00, 1.00, 0.93, 0.94, 0.97; p for trend 0.72), and no association with breast (RR 1.03), colorectal (1.01), lung (1.03), endometrial (1.15) or ovarian (1.09) cancer, all p>0.05. The paper states the associations for the individual flavonoid intakes were similar to those for total intake, and its stated conclusion is that the results DO NOT support a major role for these flavonols and flavones, or for flavonoid-rich foods, in cancer prevention. It measured no antioxidant endpoint.
  4. Hussain MS, Altamimi ASA, Afzal M, et al. Kaempferol: Paving the path for advanced treatments in aging-related diseases Experimental Gerontology. 2024;188:112389. doi:10.1016/j.exger.2024.112389.PubMedUsed to support: NARRATIVE REVIEW of preclinical work, not human evidence. Reviews kaempferol's chemistry, pharmacokinetics and proposed antioxidative, anti-inflammatory, anti-apoptotic and neuroprotective mechanisms in the context of aging-related diseases. Its own closing recommendation is that future work should refine dosing regimens, explore delivery systems and conduct comprehensive clinical trials - that is, the review itself states the clinical evidence does not yet exist. It provides mechanistic background only and does not demonstrate antioxidant, anti-inflammatory or antidiabetic effects in people. Note that it uses therapeutic language about a food constituent and asserts a favourable safety profile 'as demonstrated by preclinical and clinical studies' despite there being only one 48-person 4-week human safety trial; treat its framing with caution.
  5. Divi RL, Doerge DR Inhibition of thyroid peroxidase by dietary flavonoids Chemical Research in Toxicology. 1996;9(1):16-23. doi:10.1021/tx950076m.PubMedUsed to support: Structure-activity study of 13 commonly consumed flavonoids showing most are potent thyroid peroxidase inhibitors with IC50 values from 0.6 to 41 micromolar; kaempferol is named among the MORE POTENT compounds (with fisetin, naringenin and quercetin), all of which contain a resorcinol moiety, and its inhibition was consistent with mechanism-based, irreversible inactivation, which the authors note would be long-lasting in vivo since it could be reversed only by de novo protein synthesis. LIMITS: isolated-enzyme assay, not a human or animal outcome study, and the inhibitory concentrations are well above the ~0.1 micromolar plasma level achieved after ordinary oral kaempferol intake. Justifies replacing the page's 'theoretical ... animal data only' framing with a specific, sourced caution about concentrated high-dose supplements.
  6. DuPont MS, Day AJ, Bennett RN, Mellon FA, Kroon PA Absorption of kaempferol from endive, a source of kaempferol-3-glucuronide, in humans European Journal of Clinical Nutrition. 2004;58(6):947-54. doi:10.1038/sj.ejcn.1601916.PubMedUsed to support: Human 24-hour pharmacokinetic study (4 men, 4 women) establishing that a 9 mg oral kaempferol dose from endive produces a MEAN PEAK PLASMA CONCENTRATION OF ABOUT 0.1 MICROMOLAR at 5.8 hours, with only 1.9% of the dose excreted in urine over 24 hours, circulating almost entirely as kaempferol-3-glucuronide rather than free kaempferol. This is the number that puts the mechanism section in context: cell-culture experiments typically use micromolar to tens-of-micromolar free kaempferol, so laboratory concentrations exceed achievable human plasma levels by roughly two to three orders of magnitude, and the form in blood is a conjugate, not the aglycone used in vitro. LIMITS: n=8, single low dose, food matrix rather than a supplement, and the authors note kaempferol is actually absorbed more efficiently than quercetin.
  7. Calderón-Montaño JM, Burgos-Morón E, Pérez-Guerrero C, López-Lázaro M A review on the dietary flavonoid kaempferol Mini-Reviews in Medicinal Chemistry. 2011;11(4):298-344. doi:10.2174/138955711795305335.PubMedUsed to support: NARRATIVE REVIEW, not a clinical trial. Surveys kaempferol's distribution in edible and medicinal plants, its preclinical pharmacology (antioxidant, anti-inflammatory, antimicrobial, anticancer, cardioprotective, neuroprotective, antidiabetic and other activities), and its pharmacokinetics and safety. The pharmacological activities it catalogues are preclinical; the human associations it cites are epidemiological. Supplies background context only and should not be used as evidence that kaempferol produces any of these effects in people.
  8. Dabeek WM, Marra MV Dietary Quercetin and Kaempferol: Bioavailability and Potential Cardiovascular-Related Bioactivity in Humans Nutrients. 2019;11(10):2288. doi:10.3390/nu11102288.PubMedUsed to support: NARRATIVE REVIEW, not a clinical trial. Reviews bioavailability of quercetin and kaempferol from food, noting that glucoside conjugates (as in onions) are the best-absorbed forms and that absorbed flavonols circulate as methyl, glucuronide and sulfate metabolites. Its stated 500 mg optimal dose refers to QUERCETIN aglycone for blood pressure and inflammation, NOT to kaempferol; the review explicitly concludes that few clinical studies have examined quercetin- and kaempferol-rich plants and that studies are still needed. Supplies bioavailability context only.
  9. Chen M, Xiao J, El-Seedi HR, Woźniak KS, Daglia M, Little PJ, Weng J, Xu S Kaempferol and atherosclerosis: From mechanism to medicine Critical Reviews in Food Science and Nutrition. 2024;64(8):2157-2175. doi:10.1080/10408398.2022.2121261.PubMedUsed to support: NARRATIVE REVIEW, not a clinical trial. Reviews the antioxidant, anti-inflammatory and cardioprotective activities of kaempferol with a focus on anti-atherosclerotic mechanisms, and describes kaempferol as a possible future drug candidate rather than an established therapy. The mechanistic content is from cultured cells and animal models; the human content is epidemiological association between kaempferol-containing foods and cardiovascular risk.
  10. Akiyama M, Mizokami T, Ito H, Ikeda Y A randomized, placebo-controlled trial evaluating the safety of excessive administration of kaempferol aglycone Food Science & Nutrition. 2023;11(9):5427-5437. doi:10.1002/fsn3.3499.PubMedUsed to support: Four-week randomised, double-blind, placebo-controlled SAFETY trial (n=48; 24 kaempferol, 24 placebo) of 50 mg/day kaempferol aglycone, about five times estimated dietary intake, against a cornstarch placebo. No clinically meaningful changes versus placebo and no attributable adverse events. IMPORTANT BEYOND SAFETY: this is the only human study of kaempferol that has ever measured the outcomes this page claims. Its panel included systolic and diastolic blood pressure, C-reactive protein, total cholesterol, HDL and LDL cholesterol, triglycerides, glucose and HbA1c, at baseline, 2 weeks, 4 weeks and follow-up, and NONE of them improved on kaempferol (for example CRP 0.08 to 0.17 mg/dL on kaempferol versus 0.06 to 0.10 on placebo; total cholesterol 192.1 to 197.7 versus 190.7 to 193.5). It measured no oxidative-stress marker of any kind. LIMITS: a tolerability study in healthy adults, not powered as an efficacy trial, so the null results are uninformative rather than refuting; all four authors are employees of Otsuka Pharmaceutical Co., Ltd. This is also the highest dose of isolated kaempferol ever tested in humans, so nothing above 50 mg/day has established human safety data.
  11. Ikeda Y, Gotoh-Katoh A, Okada S, Handa S, Sato T, Mizokami T, Saito B Effect of kaempferol ingestion on physical activity and sleep quality: a double-blind, placebo-controlled, randomized, crossover trial Frontiers in Nutrition. 2024;11:1386389. doi:10.3389/fnut.2024.1386389.PubMedUsed to support: Randomised, double-blind, placebo-controlled crossover trial (n=33 city workers, 17 male and 16 female, 10 mg/day for 2 weeks with a 7-day washout, Fitbit Charge 4 accelerometry worn 23.7 h/day) reporting lower heart rate at each activity level, higher mean daily step count and distance covered, and improved sleep quality versus placebo. LIMITS: small, outcomes are device-recorded activity and self-report, three authors employed by Otsuka Pharmaceutical Co., Ltd., no independent replication, and no biochemical endpoint of any kind was measured.
  12. Okita K, Mizokami T, Akiyama M, Ikeda Y Kaempferol Reduces Cardiopulmonary Load and Muscular Damage in Repeated 400-m Sprints: A Double-Blind, Randomized, Placebo-Controlled Trial Food Science & Nutrition. 2024;12(11):9458-9468. doi:10.1002/fsn3.4506.PubMedUsed to support: Randomised, double-blind, placebo-controlled crossover trial (n=13 male university athletes, mean age 20.8 years, single 10 mg oral dose, two consecutive 400 m runs with a 90-minute interval). NULL ON RACE TIME: no significant difference in 400 m times between kaempferol and placebo. Secondary outcomes showed reduced respiratory and heart rate during the second run and reduced myoglobin and aspartate transaminase. LIMITS: n=13, single acute dose, three of the four authors are affiliated with Otsuka Pharmaceutical Co., Ltd. (the fourth is at Hokusho University) and the paper declares no conflict of interest despite this; no independent replication.
  13. Okita K, Mizokami T, Yasuda O, Ikeda Y Acute kaempferol ingestion lowers oxygen uptake during submaximal exercise and improves high-intensity exercise capacity in well-trained male athletes Physiological Reports. 2025;13(9):e70369. doi:10.14814/phy2.70369. Erratum in Physiol Rep. 2025;13(16):e70518.PubMedUsed to support: Randomised, double-blind, placebo-controlled crossover trial (n=16 well-trained male university athletes, mean VO2max 57.5 mL/kg/min, single 10 mg oral dose) reporting reduced VO2 and respiratory rate at 25/50/75% VO2max without a rise in respiratory quotient or blood lactate, and longer time to exhaustion at 100% VO2max. LIMITS: one small acute-dose crossover study; two of the four authors are affiliated with Otsuka Pharmaceutical Co., Ltd., the company behind every human kaempferol study to date, although the paper itself declares no conflict of interest; no independent replication; an erratum has been published (Physiol Rep 2025;13(16):e70518). Supports an athletic-performance signal only, not the antioxidant, anti-inflammatory or cardiovascular claims.
  14. Ikeda Y, Yamamoto M, Gotoh-Katoh A, Inoue S, Sato J Effects of kaempferol on weather-related pain: an open-label pilot study of subjective headache and other discomforts in pre-intervention and intervention periods in Japan International Journal of Biometeorology. 2025;69(10):2697-2709. doi:10.1007/s00484-025-02985-6.PubMedUsed to support: Open-label pilot, 10 mg/day kaempferol for 4 weeks; 458 enrolled and 387 analysed at over 80% adherence, reporting reduced frequency, duration and severity of weather-related symptoms (headache Cohen's d=0.61, p<0.001). LIMITS: NO CONTROL GROUP AND NO BLINDING - all outcomes are self-reported questionnaires in an uncontrolled before-and-after design, in which placebo response and regression to the mean are uncontrolled. Four of the five authors are employees of Otsuka Pharmaceutical Co., Ltd., and the study was approved by that company's own ethics committee. Included for completeness of the human record, not as evidence of efficacy.