Tribulus Terrestris (Puncture Vine / Gokshura)

Tribulus terrestris
Evidence Level
Limited
2 Clinical Trials
5 Documented Benefits
2/5 Evidence Score

Tribulus terrestris is a flowering plant used in Ayurveda (gokshura), Traditional Chinese Medicine, and Eastern European traditional medicine for libido, urinary support, and 'vitality.' Active compounds include protodioscin and other steroidal saponins. Critical: despite extensive marketing claims for testosterone enhancement, modern rigorous trials show tribulus does not meaningfully increase testosterone in healthy men — but it may improve libido and sexual function via different mechanisms. Safety note before you buy it: there are published case reports of severe liver and kidney injury in people taking tribulus supplements, including a 46-year-old man whose bilirubin peaked at 48 mg/dL with a creatinine of 7.1 and who needed plasmapheresis.

Studied Dose 450 mg/day in rugby players, 750 mg/day in postmenopausal women, 1,500 mg/day of standardized extract in the largest erectile dysfunction trial, and 6 g/day of powdered fruit for blood pressure.
Active Compound Steroidal saponins, chiefly protodioscin, together with tribulosin. An HPLC survey of commercial tribulus preparations measured 0.17 to 6.49 percent protodioscin.

Benefits

Female sexual function support (small trials, low certainty)

Multiple trials show modest improvement in libido, sexual desire, and sexual satisfaction in both men and women, including in men with low desire and in women with sexual dysfunction. In the trials in women, serum testosterone rose rather than stayed flat: free and bioavailable testosterone increased in the postmenopausal trial, and the systematic review reported a significant testosterone increase in premenopausal women after three months. So the common claim that the effect is testosterone-independent is not settled, and the nitric oxide route is a proposal rather than a demonstrated mechanism.

Erectile function scores in men diagnosed with erectile dysfunction (mixed trial results)

A meta-analysis of 8 randomized trials in men already diagnosed with erectile dysfunction found tribulus scored better than placebo on the IIEF-5 questionnaire by a mean of 3.23 points, with no difference in total testosterone between groups. The largest single trial (180 men, 12 weeks, 1,500 mg/day) was run with a Bulgarian manufacturer's product and had a company employee among its authors, and an independent 30-man trial of 800 mg/day for 30 days found no advantage over placebo at all. A separate meta-analysis of herbal products for erectile dysfunction judged the tribulus evidence insufficient. Every participant in this literature was a man under medical care for a diagnosed condition. Erectile dysfunction is a medical condition with treatable causes: see a doctor rather than self-treating it.

Urinary tract: traditional use only, and it did not hold up when measured

Sanskrit name 'gokshura' translates to 'cow hoof' (referring to fruit shape that injures cattle hooves) — and traditionally used for urinary stones, dysuria, BPH. When urinary symptoms were actually measured, in the placebo-controlled trial cited on this page, the International Prostate Symptom Score did not change on tribulus (14.4 before, 14.6 after, p = 0.67). A PubMed search for randomized human trials of tribulus alone for kidney stones returned no such trial. Set against that, two published case reports describe acute kidney injury in people taking tribulus, one of them in a man taking it specifically to prevent kidney stones. Do not take tribulus for kidney or urinary support.

Blood pressure and lipids: single small trials, different preparations

Two small randomized placebo-controlled trials have measured these. In 98 women with type 2 diabetes, 1,000 mg/day for 3 months lowered total cholesterol and LDL versus placebo, with no change in triglycerides or HDL. In prehypertensive adults, 6 g/day of powdered tribulus fruit for 2 months lowered blood pressure by 7.7/5.5 mmHg versus 1.9/0.2 mmHg on placebo. Both used preparations and doses unlike a typical standardized capsule, both were single-centre, and neither has been replicated, so this is a starting signal rather than a reason to take tribulus for your heart.

Athletic performance: does not raise testosterone or improve performance

Randomized trials in healthy young men find nothing. In 22 elite rugby league players, 450 mg/day for 5 weeks produced no difference from placebo in strength, in fat free mass, or in the urinary testosterone/epitestosterone ratio; both groups gained strength from the training itself. In 21 young men, 10 to 20 mg/kg/day for 4 weeks left testosterone, androstenedione and luteinizing hormone unchanged versus placebo. A systematic review concluded that tribulus is ineffective for raising testosterone in humans and that the marketing claims are unsubstantiated. This is one of the most over-marketed supplements relative to evidence.

Mechanism of action

1

Nitric Oxide / cGMP Pathway

Protodioscin increases nitric oxide release from corpus cavernosum endothelium in laboratory preparations. A systematic review proposed this nitric oxide effect, rather than any change in testosterone, as the plausible explanation for what people report. It has not been confirmed as the mechanism in a human study.

2

Androgen Receptor Sensitivity (not Testosterone)

Increased androgen receptor sensitivity is proposed as a way tribulus could affect libido without changing testosterone. No human study has measured androgen receptor density after tribulus, so this stays a hypothesis, and it does not explain the female trials, where serum testosterone did rise.

3

DHEA Pathway (Theoretical)

Some studies suggest modest effects on DHEA but not testosterone itself. Mechanism unclear.

4

Calcium Channel Effects (Cardiovascular)

Steroidal saponins show calcium channel modulating activity in laboratory preparations. Whether this explains the blood pressure fall seen in one small trial of powdered tribulus fruit is untested, so treat the mechanism as unconfirmed.

Clinical trials

1
Randomized trial of tribulus in postmenopausal women with hypoactive sexual desire disorder (45 randomized, 36 completed)
PubMed

Randomized, double-blind, placebo-controlled trial of tribulus 750 mg/day for 120 days in 45 healthy sexually active postmenopausal women reporting diminished libido; 36 completed, with 3 in each arm withdrawn for side effects.

45 postmenopausal women randomized, 36 completed.

On the Sexual Quotient questionnaire the tribulus group improved in desire, arousal and lubrication, pain and anorgasmia while the placebo group did not. On the Female Sexual Function Index, however, both groups improved in nearly every domain, with only lubrication improving in the tribulus group alone, so the placebo response was substantial. Free and bioavailable testosterone rose in the tribulus group. A later systematic review graded this body of evidence as very low certainty.

2
Randomized trial in elite rugby league players: no effect on strength, lean mass or the testosterone/epitestosterone ratio
PubMed

Double-blind, match-paired randomized trial of a tribulus extract at 450 mg/day versus placebo for 5 weeks of preseason heavy resistance training in 22 elite male rugby league players, 11 per group.

22 elite male Australian rugby league players, mean age 19.8 years.

Strength and fat free mass increased significantly in both groups from the training itself, with no difference between tribulus and placebo, and the urinary testosterone/epitestosterone ratio was likewise unchanged. Consistent with multiple other trials in athletes. Established that tribulus does not meaningfully increase testosterone in healthy young men.

Side effects and drug interactions

Common Potential side effects

Serious but rare: published case reports describe severe combined liver and kidney injury in people taking tribulus supplements. Most users report no problems, but stop immediately and seek medical care for jaundice, dark urine, unusual fatigue, reduced urine output or seizures.
GI distress (nausea, abdominal cramps).
Sleep disturbance / agitation in some.
Increased heart rate. In the 3-month placebo-controlled trial in men with late-onset hypogonadism, mean AST rose in the tribulus group (26.5 to 27.8, p = 0.03) and mean total PSA rose (1.4 to 1.7, p = 0.007).
Liver injury: a 46-year-old man who took tribulus supplements daily for 2 months developed severe jaundice with a peak bilirubin of 48 mg/dL and a liver biopsy showing drug-induced liver injury; he required three sessions of plasmapheresis before recovering (ACG Case Reports Journal, 2024).
Kidney injury: the same 2024 case reached a creatinine of 7.1. A separate report describes a previously healthy young man who presented with a seizure and very high aminotransferases and creatinine after only two days of a tribulus preparation he was taking to prevent kidney stones; he recovered after stopping it (Nephrology Dialysis Transplantation, 2010).
Allergic reactions rare.
Gynecomastia: a case report describes a young weight trainer who developed breast enlargement after taking a tribulus tablet as a steroid alternative for bodybuilding (Breast, 2004).
Psychosis / elevated mood — rare case reports especially in predisposed individuals.

Important Drug interactions

Diabetes medications — modest hypoglycemic effect; monitor.
Lithium — may modestly affect levels (theoretical).
Antihypertensives — additive effects.
Anticoagulants — theoretical bleeding risk.
Diuretics: additive effect, since tribulus has a mild diuretic action. Given the reported cases of liver and kidney injury, also avoid combining tribulus with other drugs or supplements that stress the liver or kidneys.
Hormone-sensitive conditions: effects on sex hormones are uncertain and the trials in women recorded a rise in testosterone, so discuss it with the doctor managing that condition before taking it.
Sympathomimetics — additive stimulating effects.

Frequently asked questions about Tribulus Terrestris (Puncture Vine / Gokshura)

What is tribulus used for?

Tribulus terrestris is a plant marketed mainly as a testosterone and libido booster for men and for athletic performance. It is also used in some traditions for libido in women and for general vitality.

Does tribulus boost testosterone?

Despite its popularity, most research shows tribulus does not meaningfully raise testosterone in healthy men. It may, however, support libido and sexual wellbeing in some people, which appears to be its more supported effect.

How much tribulus should I take?

The published trials used 450 mg/day in rugby players, 750 mg/day in postmenopausal women, 800 mg/day and 1,500 mg/day of standardized extract in erectile dysfunction trials, and 1,000 mg/day in women with diabetes. Because measured protodioscin content varies from 0.17 to 6.49 percent between products, a milligram figure on a label does not tell you how much active saponin you are getting.

Is tribulus safe?

Most people tolerate it and mild digestive upset is the usual complaint, but there are published case reports of severe liver and kidney injury in people taking tribulus, including jaundice needing plasmapheresis and a seizure with acute kidney injury. Stop taking it and seek medical care for jaundice, dark urine or reduced urine output. Product quality also varies widely: measured protodioscin content in commercial products has ranged from 0.17 to 6.49 percent. Those with hormone-sensitive conditions or on related medications should be cautious. Quality varies, so choose standardized products, and pregnant women should avoid it.

What is Tribulus Terrestris?

Tribulus terrestris is a flowering plant used in Ayurveda (gokshura), Traditional Chinese Medicine, and Eastern European traditional medicine for libido, urinary support, and 'vitality.' Active compounds include protodioscin and other steroidal saponins.

What is Tribulus Terrestris used for?

Tribulus Terrestris is researched primarily for Men's Health and Women's Health. Multiple trials show modest improvement in libido, sexual desire, and sexual satisfaction in both men and women, including in men with low desire and in women with sexual dysfunction.

What is the recommended dosage of Tribulus Terrestris?

The clinically studied dose is 450 mg/day in rugby players, 750 mg/day in postmenopausal women, 1,500 mg/day of standardized extract in the largest erectile dysfunction trial, and 6 g/day of powdered fruit for blood pressure. Always follow the product label and check with a healthcare provider for personal advice.

Is Tribulus Terrestris safe, and does it have side effects?

For most healthy adults, Tribulus Terrestris is well tolerated at studied doses. Reported effects can include: Serious but rare: published case reports describe severe combined liver and kidney injury in people taking tribulus supplements. Most users report no problems, but stop immediately and seek medical care for jaundice, dark urine, unusual fatigue, reduced urine output or seizures. It may also interact with some medications. Tribulus Terrestris is not right for everyone, so check with a healthcare provider first if you are pregnant or breastfeeding, have a medical condition, or take prescription medication.

Does Tribulus Terrestris interact with any medications?

Possible interactions include: Diabetes medications — modest hypoglycemic effect; monitor. Lithium — may modestly affect levels (theoretical). If you take prescription medication, check with a pharmacist or doctor before using it.

How strong is the scientific evidence for Tribulus Terrestris?

NutraSmarts rates the evidence for Tribulus Terrestris as Limited (2 out of 5). It is backed by 2 clinical trials and 12 cited references summarized on this page. A higher rating reflects more, larger, and better-designed human studies.

References(12 citations)

Evidence ratings on NutraSmarts are based on the totality of human clinical research, with emphasis on randomized controlled trials, meta-analyses, and systematic reviews. The references below directly support claims made throughout this page.

  1. Martimbianco ALC, Pacheco RL, Vilarino FL, Latorraca COC, Torloni MR, Riera R Tribulus Terrestris for Female Sexual Dysfunction: A Systematic Review. Revista Brasileira de Ginecologia e Obstetrícia. 2020;42(7):427-435. doi: 10.1055/s-0040-1712123.PubMedUsed to support: Systematic review of 5 randomized controlled trials in 279 women. After 1 to 3 months, premenopausal and postmenopausal women given Tribulus terrestris had significantly higher sexual function scores, and premenopausal women showed a significant rise in serum testosterone at 3 months. No serious adverse events were reported. The authors could not pool the trials because of clinical heterogeneity and graded the certainty of the evidence as very low, concluding that more trials are needed to support or refute its use.
  2. de Souza KZ, Vale FB, Geber S Efficacy of Tribulus terrestris for the treatment of hypoactive sexual desire disorder in postmenopausal women: a randomized, double-blinded, placebo-controlled trial. Menopause. 2016;23(11):1252-1256..PubMedUsed to support: Randomized, double-blind, placebo-controlled trial in 45 postmenopausal women reporting diminished libido, of whom 36 completed 120 days on 750 mg/day. On the Sexual Quotient questionnaire, desire, arousal and lubrication, pain and anorgasmia improved in the tribulus group and not in the placebo group. On the Female Sexual Function Index both groups improved in every domain except lubrication, which improved only on tribulus, so much of the change was matched by placebo. Free and bioavailable testosterone rose in the tribulus group. Three women in each arm were withdrawn because of side effects.
  3. GamalEl Din SF, Abdel Salam MA, Mohamed MS, Ahmed AR, Motawaa AT, Saadeldin OA, Elnabarway RR Tribulus terrestris versus placebo in the treatment of erectile dysfunction and lower urinary tract symptoms in patients with late-onset hypogonadism: A placebo-controlled study. Urologia. 2019;86(2):74-78..PubMedUsed to support: Placebo-controlled trial in 70 aging men with late-onset hypogonadism who had erectile dysfunction and lower urinary tract symptoms; 35 took Tribulus terrestris three times daily for 3 months and 35 took placebo. In the tribulus group the erectile function score rose from 10.7 to 16.1 and total testosterone rose from 2.2 to 2.7, both reported as before-and-after changes within that group. Lower urinary tract symptoms did not improve: the International Prostate Symptom Score was 14.4 before and 14.6 after (p = 0.67). Mean AST rose from 26.5 to 27.8 (p = 0.03) and mean total PSA rose from 1.4 to 1.7 (p = 0.007).
  4. Mohy-Ud-Din N, Jonassaint N Severe Liver and Renal Injury From Tribulus Terrestris. ACG Case Reports Journal. 2024;11(2):e01267. doi: 10.14309/crj.0000000000001267.PubMedUsed to support: Case report of a 46-year-old man who took tribulus supplements daily for 2 months and developed severe jaundice requiring hospital admission, with a peak total bilirubin of 48 mg/dL, a creatinine of 7.1 and a liver biopsy showing drug-induced liver injury. He needed three sessions of plasmapheresis and recovered. A single case cannot establish how often this happens, but it documents that tribulus alone can precipitate combined liver and kidney failure in a previously well adult.
  5. Talasaz AH, Abbasi MR, Abkhiz S, Dashti-Khavidaki S Tribulus terrestris-induced severe nephrotoxicity in a young healthy male. Nephrology, Dialysis, Transplantation. 2010;25(11):3792-3. doi: 10.1093/ndt/gfq457.PubMedUsed to support: Case report of a young healthy man who took a Tribulus terrestris preparation to prevent kidney stone formation and presented with a seizure and very high serum aminotransferases and creatinine after only two days. His symptoms improved and his liver enzymes normalized after he stopped the herb. The exposure that caused the injury was the same use, urinary and stone prevention, for which the plant is traditionally recommended.
  6. Rogerson S, Riches CJ, Jennings C, Weatherby RP, Meir RA, Marshall-Gradisnik SM The effect of five weeks of Tribulus terrestris supplementation on muscle strength and body composition during preseason training in elite rugby league players. Journal of Strength and Conditioning Research. 2007;21(2):348-53. doi: 10.1519/R-18395.1.PubMedUsed to support: Double-blind randomized trial in 22 elite male Australian rugby league players, match-paired to 450 mg/day of a Tribulus terrestris extract or placebo for 5 weeks of preseason heavy resistance training. Strength and fat free mass rose significantly in both groups from the training itself, with no difference between tribulus and placebo, and the urinary testosterone to epitestosterone ratio was unchanged. The authors concluded that tribulus does not produce the strength or lean mass gains manufacturers claim.
  7. Neychev VK, Mitev VI The aphrodisiac herb Tribulus terrestris does not influence the androgen production in young men. Journal of Ethnopharmacology. 2005;101(1-3):319-23. doi: 10.1016/j.jep.2005.05.017.PubMedUsed to support: Randomized trial in 21 healthy men aged 20 to 36 assigned to 20 mg/kg/day, 10 mg/kg/day, or placebo for 4 weeks. Serum testosterone, androstenedione and luteinizing hormone were measured at six timepoints and showed no significant difference between the tribulus groups and controls, with all values inside the normal range. A second null trial, at doses well above typical commercial servings.
  8. Qureshi A, Naughton DP, Petroczi A A systematic review on the herbal extract Tribulus terrestris and the roots of its putative aphrodisiac and performance enhancing effect. Journal of Dietary Supplements. 2014;11(1):64-79. doi: 10.3109/19390211.2014.887602.PubMedUsed to support: Systematic review searching seven databases plus the patent database, in which 11 records met the inclusion criteria: randomized controlled trials in healthy humans and animal studies, along with one patent application. Trials varied too much in duration and dose to pool. Some animal studies showed a testosterone rise, but in humans an increase was seen only when tribulus was given as part of a combined supplement. The authors concluded that tribulus is ineffective for increasing testosterone in humans and that marketing claims are unsubstantiated, and proposed that nitric oxide release, not any hormone change, explains the physiological responses people report.
  9. Suharyani S, Amanda B, Angellee J, William W, Hariyanto TI, I'tishom R Tribulus terrestris for management of patients with erectile dysfunction: a systematic review and meta-analysis of randomized trials. International Journal of Impotence Research. 2026;38(1):11-18. doi: 10.1038/s41443-025-01086-7. Epub 2025 May 13.PubMedUsed to support: Systematic review and meta-analysis of 8 randomized studies in men diagnosed with erectile dysfunction. Tribulus outperformed placebo on the IIEF-5 (mean difference 3.23 points, 95% CI 1.89 to 4.58) and on the IIEF-15 (mean difference 14.44, 95% CI 5.75 to 23.14), while total testosterone did not differ between groups and adverse event rates were similar. Every participant had a diagnosed condition and was under medical care, so this does not describe what happens in men without that diagnosis.
  10. Ho CY, Hsu CH, Chien TJ Herbal dietary supplements for erectile dysfunction: A systematic review and meta-analysis of randomized-controlled trials. Journal of Traditional and Complementary Medicine. 2026;16(1):109-120. doi: 10.1016/j.jtcme.2025.11.001.PubMedUsed to support: Systematic review and meta-analysis of 14 randomized trials in 1,227 men with erectile dysfunction, comparing herbal supplements with placebo or no intervention. Saffron and ginseng carried the clearest results across erectile function, orgasmic function and satisfaction. The authors state that evidence for other agents, naming Tribulus terrestris and maca, remains insufficient and that larger, higher-quality trials with longer follow-up are needed.
  11. Ganzera M, Bedir E, Khan IA Determination of steroidal saponins in Tribulus terrestris by reversed-phase high-performance liquid chromatography and evaporative light scattering detection. Journal of Pharmaceutical Sciences. 2001;90(11):1752-8. doi: 10.1002/jps.1124.PubMedUsed to support: Analytical study that developed an HPLC method for the marker saponin protodioscin and applied it to plant material and to commercial products. Saponin composition differed significantly with the plant part used and the country of origin, and protodioscin content across market products ranged from 0.17 to 6.49 percent, a 38-fold spread. Two tribulus products at the same milligram serving can therefore deliver very different amounts of the compound the trials were about.
  12. Jameel JK, Kneeshaw PJ, Rao VS, Drew PJ Gynaecomastia and the plant product "Tribulis terrestris". The Breast. 2004;13(5):428-30. doi: 10.1016/j.breast.2003.10.013.PubMedUsed to support: Case report of a young weight trainer who developed gynaecomastia after taking a tribulus herbal tablet as an alternative to anabolic steroids for bodybuilding. It is the published source behind the gynaecomastia entry in this page's side effect list.