Lactobacillus fermentum CECT5716 (Lc40 / Hereditum®)

Limosilactobacillus fermentum CECT5716 (formerly Lactobacillus fermentum CECT5716)
Evidence Level
Limited
3 Clinical Trials
6 Documented Benefits
2/5 Evidence Score

Lactobacillus fermentum CECT5716 (commercialized as Lc40 and Hereditum®) is a probiotic strain originally isolated from human breast milk. It has been studied mainly in breastfeeding women, in trials looking at lactational mastitis (a breast infection during nursing) and breast pain. This is a specific clinical use in a medical population, not a general wellness benefit. Multiple randomized trials show meaningful reductions in mastitis recurrence and breast pain when taken daily during lactation. Separate trials in formula-fed infants reported fewer gastrointestinal infections, but that is a different population and does not apply to an adult taking a capsule. The evidence comes almost entirely from the strain's manufacturer, and in 2017 the European Food Safety Authority reviewed it and concluded that a cause and effect relationship between this strain and reduced mastitis risk had not been established. There is no evidence for general adult use beyond lactation.

Studied Dose 3×10⁹ CFU/day in the lactation trials (16 weeks in the 625-woman prevention trial, 3 weeks in treatment trials). Consumer Hereditum supplies about 1 to 3 billion CFU/day.
Active Compound Limosilactobacillus (Lactobacillus) fermentum CECT5716; trademarked HEREDITUM®/Lc40 (Biosearch Life).

Benefits

Studied for breast health during breastfeeding

In lactating women, one manufacturer-sponsored randomized trial (625 enrolled, 291 completing the 16 week course) reported a lower incidence rate of clinical mastitis in the probiotic group (18 episodes versus 40, rate 0.130 versus 0.263). An independent 2017 European Food Safety Authority review concluded the cause and effect relationship was not established. This is a clinical outcome studied in nursing women, not a general benefit.

Studied against antibiotics in lactating women

In one open-label trial (352 lactating women) that was not placebo-controlled, women taking this strain for 3 weeks improved similarly to women given antibiotics. It was an unblinded comparison in women already under medical care for a breast infection, not evidence that a supplement should replace medical treatment.

Reduced breast pain during lactation

In manufacturer-run trials of lactating women with painful breastfeeding, this strain was associated with lower breast pain scores versus placebo. The finding comes from a narrow clinical group under care, not from general use.

Studied in infant formula (a separate population)

In infant formula trials, this strain was linked to fewer gastrointestinal infections in babies. That evidence is in infants fed a supplemented formula, a different population that does not transfer to an adult taking a capsule. The cited safety trial measured gastrointestinal infections, not respiratory ones.

Originally isolated from human milk

The strain was originally isolated from healthy breast milk. It has a good safety record in trials of lactating women and infants. A proposed European health claim for reducing mastitis risk was reviewed and rejected in 2017.

Antimicrobial peptide production

In laboratory tests, this strain produces compounds active against Staphylococcus bacteria. This is a proposed mechanism observed in vitro, not a demonstrated clinical benefit.

Mechanism of action

1

Mammary microbiota niche specialization (breast milk origin)

Originally isolated from healthy breast milk — the strain is naturally adapted to the mammary environment. Niche specialization is the proposed basis for selective benefit at this site vs other Lactobacillus strains isolated from gut or vaginal sources.

2

Staphylococcus competitive exclusion

Trial data reported significantly lower Staphylococcus spp. load in breast milk of the probiotic group. Staphylococcus is the main causal agent of mastitis. Competitive exclusion via niche occupation, antimicrobial peptide production, and lactic acid / low-pH maintenance are the proposed mechanisms.

3

Antimicrobial peptide production

Bacteriocin-like substances active against Staphylococcus species are produced by L. fermentum CECT5716 in vitro. This is a foundational mechanism for the Staphylococcus-load reduction observed clinically.

4

Lactic acid and low-pH maintenance

Lactobacillus species produce lactic acid lowering local pH — a foundational antimicrobial mechanism that disadvantages alkaline-tolerant pathogens including some Staphylococcus species.

5

Mammary microbiota dysbiosis correction

Painful breastfeeding and mastitis are associated with dysbiosis of the mammary microbiota — Staphylococcus overgrowth at the expense of normal commensals. Restoring commensal balance is the proposed broader mechanism beyond direct Staphylococcus suppression.

Clinical trials

1
Mastitis Prevention 625-Women Clinical Trial

Clinical evidence on Lactobacillus fermentum CECT5716 (Lc40 / Hereditum®) for the indications and outcomes described.

625 women

Hurtado JA et al. 2017, Breastfeeding Medicine, NCT02203877, PROLAC Group multicenter investigation. Randomized double-blind placebo-controlled multicenter trial in 625 women (291 completed 16-week treatment) recruited 1-6 days post-childbirth. Probiotic: 3×10⁹ CFU/day L. fermentum CECT5716 vs maltodextrin placebo for 16 weeks. Clinical mastitis incidence rate 0.130 (probiotic) vs 0.263 (control), p=0.021 — 51% reduction. Staphylococcus load in breast milk was lower in the probiotic group. Authored by staff of the strain's manufacturer; an independent 2017 European Food Safety Authority review found the cause and effect relationship for reducing mastitis risk was not established.

2
Hereditum® Mastitis Treatment Dose-Ranging Clinical Trial
PubMed

4-arm dose-ranging trial (3×10⁹, 6×10⁹, 9×10⁹ CFU/day, plus placebo) for 3 weeks in women with painful breastfeeding.

Clinical population described in trial publication.

4-arm dose-ranging trial (3×10⁹, 6×10⁹, 9×10⁹ CFU/day, plus placebo) for 3 weeks in women with painful breastfeeding. Compared with baseline, Staphylococcus counts fell within the probiotic groups while the placebo group stayed similar, and breast pain scores were lower in the probiotic groups. No dose-response was seen: the three doses behaved alike. This is a manufacturer-run trial in lactating women with painful breastfeeding (Maldonado-Lobon 2015).

3
Australian Trial Protocol (design only, no results)
PubMed

Clinical evidence on Lactobacillus fermentum CECT5716 (Lc40 / Hereditum®) for the indications and outcomes described.

Clinical population described in trial publication.

Bond DM, Morris JM, Nassar N. 2017, BMC Pregnancy and Childbirth, PMID 28525984. This is a published study protocol only (the APProve trial, planning 600 women), describing a design to test whether a probiotic prevents mastitis. It reports no participants and no results, so it provides no efficacy evidence.

Side effects and drug interactions

Common Potential side effects

Generally extremely well-tolerated; isolated from breast milk = naturally human-adapted strain.
Mild GI upset (rare; transient).
Pregnancy/lactation: especially designed for lactating women — extensive 16-week safety record.
Long-term safety: 16-week multicenter RCT in 625 women supportive.
Allergic reactions (rare).
Severely immunocompromised individuals: caution. Live Lactobacillus probiotics have rare case reports of bacteraemia in vulnerable patients, and this is a live organism that needs proper storage to stay viable.
Industry-sponsorship (Biosearch Life Hereditum®) — important context.

Important Drug interactions

Antibiotics: take 2-3 hours apart from L. fermentum CECT5716 dose.
This strain is not a treatment for mastitis. Anyone with a suspected breast infection should see a clinician rather than rely on a supplement.
Most medications: well-tolerated combination profile.
Immunosuppressants: caution.
Other probiotics: compatible.
Anticoagulants: no interactions documented.
Hormonal contraceptives: no interactions documented (relevant to postpartum population).

Frequently asked questions about Lactobacillus fermentum CECT5716 (Lc40 / Hereditum®)

What is Lactobacillus fermentum CECT5716?

Lactobacillus fermentum CECT5716 (commercialized as Lc40 and Hereditum®) is a probiotic strain originally isolated from human breast milk. It has been studied mainly in breastfeeding women, in trials looking at lactational mastitis (a breast infection during nursing) and breast pain.

What is Lactobacillus fermentum CECT5716 used for?

Lactobacillus fermentum CECT5716 is researched primarily for Women's Health. In lactating women, one manufacturer-sponsored randomized trial (625 enrolled, 291 completing the 16 week course) reported a lower incidence rate of clinical mastitis in the probiotic group (18 episodes versus 40, rate 0.130 versus 0.263).

What is the recommended dosage of Lactobacillus fermentum CECT5716?

The clinically studied dose is 3×10⁹ CFU/day in the lactation trials (16 weeks in the 625-woman prevention trial, 3 weeks in treatment trials). Consumer Hereditum supplies about 1 to 3 billion CFU/day. Always follow the product label and check with a healthcare provider for personal advice.

Is Lactobacillus fermentum CECT5716 safe, and does it have side effects?

For most healthy adults, Lactobacillus fermentum CECT5716 is well tolerated at studied doses. Reported effects can include: Generally extremely well-tolerated; isolated from breast milk = naturally human-adapted strain. Mild GI upset (rare; transient). It may also interact with some medications. Lactobacillus fermentum CECT5716 is not right for everyone, so check with a healthcare provider first if you are pregnant or breastfeeding, have a medical condition, or take prescription medication.

Does Lactobacillus fermentum CECT5716 interact with any medications?

Possible interactions include: Antibiotics: take 2-3 hours apart from L. fermentum CECT5716 dose. This strain is not a treatment for mastitis. Anyone with a suspected breast infection should see a clinician rather than rely on a supplement. If you take prescription medication, check with a pharmacist or doctor before using it.

How strong is the scientific evidence for Lactobacillus fermentum CECT5716?

NutraSmarts rates the evidence for Lactobacillus fermentum CECT5716 as Limited (2 out of 5). It is backed by 3 clinical trials and 3 cited references summarized on this page. A higher rating reflects more, larger, and better-designed human studies.

References(3 citations)

Evidence ratings on NutraSmarts are based on the totality of human clinical research, with emphasis on randomized controlled trials, meta-analyses, and systematic reviews. The references below directly support claims made throughout this page.

  1. Arroyo R, Martin V, Maldonado A, Jimenez E, Fernandez L, Rodriguez JM Treatment of infectious mastitis during lactation: antibiotics versus oral administration of Lactobacilli isolated from breast milk Clinical Infectious Diseases. 2010;50(12):1551-8. doi: 10.1086/652763.PubMedUsed to support: In 352 lactating women with infectious mastitis (L. fermentum arm n=124), those taking L. fermentum CECT5716 for 3 weeks improved more and had fewer recurrences than women given antibiotics. Limits: an open-label trial with no placebo group, comparing the strain against antibiotics in women under medical care, and the strain originates from a lab linked to the manufacturer.
  2. Maldonado-Lobon JA, Diaz-Lopez MA, Carputo R, Duarte P, Diaz-Ropero MP, Valero AD, Sanudo A, Sempere L, Ruiz-Lopez MD, Banuelos O, et al. Lactobacillus fermentum CECT 5716 Reduces Staphylococcus Load in the Breastmilk of Lactating Mothers Suffering Breast Pain: A Randomized Controlled Trial Breastfeeding Medicine. 2015;10(9):425-32. doi: 10.1089/bfm.2015.0070.PubMedUsed to support: In lactating women with painful breastfeeding, 3 weeks of L. fermentum CECT5716 lowered breast pain scores versus placebo, and Staphylococcus counts fell within the probiotic groups from baseline while the placebo group stayed similar. Limits: a small manufacturer-run trial using a bacterial-count surrogate rather than clinical mastitis, with no dose-response across the three doses.
  3. Gil-Campos M, Lopez MA, Rodriguez-Benitez MV, Romero J, Roncero I, Linares MD, Maldonado J, Lopez-Huertas E, Berwind R, Ritzenthaler KL, et al. Lactobacillus fermentum CECT 5716 is safe and well tolerated in infants of 1-6 months of age: a randomized controlled trial Pharmacological Research. 2012;65(2):231-8. doi: 10.1016/j.phrs.2011.11.016.PubMedUsed to support: An infant formula containing L. fermentum CECT5716 was safe and well tolerated in babies 1 to 6 months old, with fewer gastrointestinal infections than standard formula. Limits: this is evidence in formula-fed infants, a different population from an adult taking a capsule, and the trial was powered for safety, not efficacy, and did not show a respiratory benefit.