Zylaria® (Xylaria nigripes Mycelium Extract)

Xylaria nigripes
Evidence Level
Preliminary
2 Clinical Trials
4 Documented Benefits
1/5 Evidence Score

Zylaria® (NuLiv Science) is a branded blend of Xylaria nigripes mycelium extract with purified fractions of Panax notoginseng root and Cuscuta chinensis seed. Xylaria nigripes is a termite-nest-associated fungus used in Traditional Chinese Medicine as Wu Ling Shen (乌靈参) for sleep and emotional balance. The clinical data come from the parent fungus, not from Zylaria®. The placebo-controlled trial of the related Wuling capsule in insomnia found no significant difference from placebo on any sleep measure, and a small pilot trial in adults with epilepsy reported a difference in depressive symptoms whose confidence interval still crossed zero. The fungus contains amino acids, polysaccharides, and small molecules hypothesized to support GABAergic signaling and the HPA-axis stress response. NuLiv Science states that Zylaria® was tested in a human trial, but that study has never been published and does not appear in public trial registries, so its size, design and results cannot be checked. Everything here therefore rests on parent-species research and the supplier's specification.

Studied Dose 500 mg/day (NuLiv Science spec). The published trials did not use Zylaria®: the insomnia trial used Wuling capsule at about 3 g/day of Xylaria nigripes mycelial powder, roughly six times the branded serving and a different preparation.
Active Compound Xylaria nigripes mycelium extract with purified fractions of Panax notoginseng root and Cuscuta chinensis seed (NuLiv Science spec); constituents include free amino acids (notably GABA and related precursors), polysaccharides, and small molecules linked to TCM "Wu Ling Shen."

Benefits

Sleep: no benefit over placebo in the controlled trial

The one placebo-controlled insomnia trial did not support this. Ninety-four adults took Wuling capsule and 92 took placebo for four weeks; both groups improved on the Pittsburgh Sleep Quality Index, but there was no significant difference between the groups, and clinical global impression and quality-of-life scores did not differ either. The authors concluded that none of the variables tested separated from placebo. Zylaria® is marketed for adults seeking gentle botanical sleep support alongside good sleep habits, but that is a marketing position and not a demonstrated effect.

Mood in one small pilot trial

Wu Ling Shen has a long history in Traditional Chinese Medicine for emotional balance and calm. The modern evidence is a single 12-week pilot trial in adults with epilepsy, a diagnosed condition managed by a doctor. Of 104 people randomised, 81 finished; the share rated as responding on depressive symptoms was 51.3% on Xylaria nigripes and 35.7% on placebo. The paper reports that gap as 0.15 with a 95% confidence interval of -0.06 to 0.37, an interval that includes zero, alongside P=0.002. Those two figures cannot both be right, and the interval is the more cautious reading. Nothing cited here measured stress or anxiety, and nothing was tested in healthy adults.

Next-day alertness has not been tested

This describes a product category rather than anything measured for Zylaria®. No cited study compared it with a sedative-hypnotic medicine or measured next-day alertness, and the safety notes on this page flag possible drowsiness, so freedom from daytime grogginess cannot be claimed.

Adjunct to sleep-hygiene routines

Zylaria® fits within a broader sleep-support strategy that includes consistent sleep timing, light exposure management, caffeine timing, and stress regulation. It is intended as a supportive ingredient rather than a stand-alone insomnia therapeutic.

Mechanism of action

1

GABA precursor and GABAergic signaling support

Xylaria nigripes preparations are enriched in free amino acids including GABA itself and related precursors. Preclinical work suggests GABAergic system modulation, potentially contributing to calming and sleep-promoting effects, although direct human neuropharmacology data remains limited.

2

HPA-axis and stress-response modulation

Traditional and emerging mechanistic work positions Xylaria nigripes within the family of adaptogen-like botanicals that may help modulate the hypothalamic-pituitary-adrenal axis during periods of perceived stress. This is a hypothesis: no study cited on this page measured cortisol or any other marker of HPA-axis activity.

3

Polysaccharide and small-molecule bioactives

Xylaria nigripes contains polysaccharides and small-molecule constituents that have been characterized in natural product chemistry studies. Several have been associated with neuroprotective or anti-inflammatory activity in preclinical assays, providing additional mechanistic rationale.

Clinical trials

1
Xylaria nigripes and depressive symptoms in adults with epilepsy: pilot multicenter RCT

Multicenter, randomized, double-blind, placebo-controlled trial of Xylaria nigripes (Wu Ling capsule) vs placebo in 104 adults with epilepsy and depressive symptoms over 12 weeks. Outcomes: Hamilton Depression Rating Scale, response rates. Published in Seizure.

104 adults with epilepsy and depressive symptoms randomised, 81 completed; 12-week intervention. A diagnosed disease population under medical care, not healthy adults.

The authors describe this as a pilot study. The primary endpoint, total effective rate for depressive symptoms, was 51.3% on Xylaria nigripes and 35.7% on placebo among the 81 people who completed. The paper gives that difference as 0.15 with a 95% confidence interval of -0.06 to 0.37, which includes zero, while also reporting P=0.002; the two statements are inconsistent and the confidence interval is the more cautious reading. No difference was found in seizure frequency or severity. Quality of life and the seizure worry subscale improved more on treatment. The product tested was generic Xylaria nigripes, not Zylaria®.

2
Wuling capsule (Xylaria nigripes) for insomnia in Chinese adults: multicenter RCT with a negative result

Multicenter, randomized, double-blind, placebo-controlled trial of Wuling capsule (Xylaria nigripes mycelial powder) at 3 g/day vs placebo in 186 Chinese adults with insomnia over 4 weeks, with 2-week follow-up. Outcomes: Pittsburgh Sleep Quality Index, clinical global impression, WHOQOL-BREF quality of life, safety. Published in Journal of Ethnopharmacology.

186 Chinese adults with insomnia; 4-week intervention plus 2-week follow-up.

Wuling capsule did not beat placebo. Both groups improved on the global Pittsburgh Sleep Quality Index against their own baseline, but there was no significant difference between the Wuling and placebo groups. Clinical global impression scores were similar to placebo at every week, and all four WHOQOL-BREF quality-of-life components improved in both groups with no difference between them. The authors concluded that none of the variables tested differed from placebo control. Adverse events were 10.10% on Wuling and 6.73% on placebo, not a significant difference. This is the most directly relevant placebo-controlled trial for Xylaria nigripes in sleep, and it is negative.

Side effects and drug interactions

Common Potential side effects

Generally well tolerated in published Xylaria nigripes trials, though those tested the parent material rather than the branded blend; no human safety data on Zylaria® itself has been published.
Mild GI symptoms (nausea, abdominal discomfort) possible at higher doses.
Theoretical drowsiness — assess individual response before driving or operating machinery.
Pregnancy and lactation safety data are limited; avoid use without clinician guidance.
Discontinue if allergic reaction or unexpected mood change develops.

Important Drug interactions

Sedatives and CNS depressants (benzodiazepines, sleep medications) — theoretical additive sedation; use cautiously.
Antidepressants and antiepileptics — coordinate use with prescribing clinician, especially in the context of epilepsy or mood disorders.
Alcohol — may potentiate any sedative effects; limit during use.
GABAergic agents — theoretical additive effect; discuss with clinician before combining.

Frequently asked questions about Zylaria® (Xylaria nigripes Mycelium Extract)

What is Zylaria?

Zylaria® (NuLiv Science) is a branded blend of Xylaria nigripes mycelium extract with purified fractions of Panax notoginseng root and Cuscuta chinensis seed. Xylaria nigripes is a termite-nest-associated fungus used in Traditional Chinese Medicine as Wu Ling Shen (乌靈参) for sleep and emotional balance.

What is Zylaria used for?

Zylaria is researched primarily for Mood & Mental Health. The one placebo-controlled insomnia trial did not support this. Ninety-four adults took Wuling capsule and 92 took placebo for four weeks; both groups improved on the Pittsburgh Sleep Quality Index, but there was no significant difference b…

What is the recommended dosage of Zylaria?

The clinically studied dose is 500 mg/day (NuLiv Science spec). The published trials did not use Zylaria®: the insomnia trial used Wuling capsule at about 3 g/day of Xylaria nigripes mycelial powder, roughly six times the branded serving and a different preparation. Always follow the product label and check with a healthcare provider for personal advice.

Is Zylaria safe, and does it have side effects?

For most healthy adults, Zylaria is well tolerated at studied doses. Reported effects can include: Generally well tolerated in published Xylaria nigripes trials, though those tested the parent material rather than the branded blend; no human safety data on Zylaria® itself has been published. Mild GI symptoms (nausea, abdominal discomfort) possible at higher doses. It may also interact with some medications. Zylaria is not right for everyone, so check with a healthcare provider first if you are pregnant or breastfeeding, have a medical condition, or take prescription medication.

Does Zylaria interact with any medications?

Possible interactions include: Sedatives and CNS depressants (benzodiazepines, sleep medications) — theoretical additive sedation; use cautiously. Antidepressants and antiepileptics — coordinate use with prescribing clinician, especially in the context of epilepsy or mood disorders. If you take prescription medication, check with a pharmacist or doctor before using it.

How strong is the scientific evidence for Zylaria?

NutraSmarts rates the evidence for Zylaria as Preliminary (1 out of 5). It is backed by 2 clinical trials and 2 cited references summarized on this page. A higher rating reflects more, larger, and better-designed human studies.

References(2 citations)

Evidence ratings on NutraSmarts are based on the totality of human clinical research, with emphasis on randomized controlled trials, meta-analyses, and systematic reviews. The references below directly support claims made throughout this page.

  1. Peng WF, Wang X, Hong Z, Zhu GX, Li BM, Li Z, Ding MP, Geng Z, Jin Z, Miao L, Wu LW, Zhan SK. The anti-depression effect of Xylaria nigripes in patients with epilepsy: A multicenter randomized double-blind study. Seizure. 2015;29:26-33. doi: 10.1016/j.seizure.2015.03.014.PubMedUsed to support: Pilot multicenter RCT: 104 adults with epilepsy randomised, 81 completed. Total effective rate for depressive symptoms 51.3% versus 35.7% on placebo, reported as a difference of 0.15 with a 95% confidence interval of -0.06 to 0.37 that includes zero, alongside P=0.002. Tested generic Xylaria nigripes in a disease population, not Zylaria®.
  2. Lin Y, Wang XY, Ye R, Hu WH, Sun SC, Jiao HJ, Song XH, Yuan ZZ, Zheng YY, Zheng GQ, He JC. Efficacy and safety of Wuling capsule, a single herbal formula, in Chinese subjects with insomnia. J Ethnopharmacol. 2013;145(1):320-7. doi: 10.1016/j.jep.2012.11.009.PubMedUsed to support: Multicenter placebo-controlled RCT in 186 Chinese adults with insomnia. Wuling capsule (Xylaria nigripes about 3 g/day) improved sleep against baseline but showed no significant difference from placebo on the Pittsburgh Sleep Quality Index, clinical global impression, or quality of life. Cited here as the negative sleep result it is, not as support for a sleep claim.