Phytase

Phytase / myo-inositol hexakisphosphate phosphohydrolase (EC 3.1.3.8/26)
Evidence Level
Moderate
2 Clinical Trials
4 Documented Benefits
3/5 Evidence Score

Phytase is an enzyme that hydrolyzes phytic acid (phytate / inositol hexaphosphate) — a major mineral-binding 'antinutrient' found in grains, legumes, nuts, and seeds. Phytate binds calcium, iron, zinc, magnesium, and other essential minerals and reduces how much of them the body can absorb. Humans produce minimal endogenous phytase, making us particularly susceptible to phytate-induced mineral deficiencies on grain-heavy diets. Human absorption studies show that phytase raises iron and zinc absorption when it is added to a high phytate meal. Those studies treated the food itself, either during preparation or immediately before eating, so the same enzyme taken as a capsule is a reasonable but so far untested extension.

Studied Dose Labels commonly list 500 to 1,500 FTU per serving. In absorption studies, 20 to 320 phytase units per 100 g of flour improved iron absorption, with the enzyme mixed into food rather than a capsule.
Active Compound Phytase enzyme (typically Aspergillus niger-derived) measured in FTU (Phytase Units) or PU

Benefits

Improved iron, zinc, calcium, and magnesium absorption from plant foods

Phytate is the main dietary inhibitor of iron and zinc absorption from plant foods, and it also binds calcium and magnesium. How much absorption is lost depends on the phytate to mineral ratio of the meal rather than on any fixed percentage. Phytase hydrolyzes phytate, freeing these minerals for absorption. Multiple in vitro and in vivo studies confirm phytase pre-treatment of grain meals significantly improves iron and zinc bioavailability. In those studies the enzyme was mixed into the food, either during preparation or immediately before eating, rather than swallowed as a separate capsule, so the capsule format itself has not been tested. The findings apply most directly to people whose meals are built on grains and legumes.

Critical for plant-based diets and vegetarian populations

Vegetarians and vegans typically have 2–3× higher phytate intake than omnivores due to higher consumption of grains, legumes, nuts, and seeds. In controlled feeding work, a lactoovovegetarian diet lowered zinc absorption efficiency by about 21 percent compared with an omnivorous diet, although the women in that study still maintained zinc balance on both diets. Phytase, along with traditional methods of phytate reduction such as soaking, sprouting and fermenting, lowers the phytate load of these meals. The one long trial to measure mineral status, 23 weeks in South African children, used a powder that combined phytase with iron, zinc and vitamin C stirred into porridge and did improve iron and zinc status, so it cannot show what phytase does on its own.

Digestive comfort is not established

Phytase is often included in digestive enzyme blends sold for comfort after grain-heavy meals, but none of the studies cited here measured bloating, fullness or any other symptom, and no trial has tested phytase on its own against functional dyspepsia, which is a diagnosed condition. Treat digestive comfort as an untested claim.

Inositol release, chemistry rather than a proven benefit

Phytase hydrolysis of phytate releases free myo-inositol, the same compound sold on its own as an inositol supplement. The amount freed from a single meal is small, and nothing cited here measures inositol levels, blood sugar, mood or reproductive outcomes from phytase, so this is a point of chemistry rather than a health benefit.

Mechanism of action

1

Sequential phosphate cleavage from inositol hexaphosphate

Phytase enzymes cleave phosphate groups from phytic acid (inositol hexaphosphate, IP6) in stepwise fashion: IP6 → IP5 → IP4 → IP3 → IP2 → IP1 → free inositol + 6 phosphates. Each cleavage step releases a phosphate group and reduces the molecule's mineral-binding capacity. Lower IP forms (IP1-3) have minimal mineral-binding effect, so even partial phytase activity provides substantial mineral liberation.

2

Acid-active for stomach efficacy

Effective supplemental phytases (typically from Aspergillus niger) are acid-active (optimal pH 2.5–5), allowing them to begin working immediately in the stomach. This is critical because most mineral absorption occurs in the proximal small intestine — phytase must complete its work before food leaves the stomach for maximum benefit.

3

Synergistic with traditional phytate-reduction methods

Traditional food preparation (soaking, sprouting, fermentation, sourdough leavening) reduces phytate content of grains and legumes via endogenous plant phytase activation and microbial fermentation. Supplemental phytase complements these methods, particularly for unfermented grain products (modern bread, pasta, rice) where phytate remains largely intact.

Clinical trials

1
Phytase and Iron Absorption from Cereal Meals — Stable Isotope Studies
PubMed

Iron absorption measured in adults from nine roller-dried cereal porridges made with and without an added phytase that fully degraded the phytic acid during manufacture, using an extrinsic radioiron label. (Hurrell RF, Reddy MB, Juillerat MA, Cook JD. Am J Clin Nutr. 2003;77(5):1213-1219. PMID 12716674)

Adults consuming cereal meals.

Removing the phytic acid raised iron absorption from rice porridge from 1.73 percent to 5.34 percent and from wheat porridge from 0.99 percent to 11.54 percent. The gain largely disappeared when the wheat porridge was made with milk instead of water, so how much is gained depends on what else is in the meal.

2
Phytase and Zinc Absorption from Fortified Porridges
PubMed

Five zinc absorption studies using the double isotopic tracer ratio method in young adults eating maize or sorghum porridges fortified with zinc. (Brnic M, Wegmuller R, Zeder C, Senti G, Hurrell RF. J Nutr. 2014;144(9):1467-1473. PMID 24966411)

Young adults.

Adding phytase to the maize porridge immediately before it was eaten, or using phytase to remove the phytate during preparation, both raised fractional zinc absorption by more than 80 percent, with P below 0.001 for each. This is single-meal absorption, not zinc status over weeks.

Side effects and drug interactions

Common Potential side effects

Generally well tolerated as a food-grade enzyme
Allergic reactions to fungal source in sensitized individuals
People with hemochromatosis or another iron overload condition should avoid phytase, since it is taken specifically to increase iron absorption from food. It also should not be combined with high-dose mineral supplements at the same time — phytase liberates minerals from food matrix, but excess concurrent mineral intake can cause GI upset

Important Drug interactions

Mineral supplements (iron, zinc, calcium) — synergistic effect; phytase may enhance absorption
Bisphosphonates — separate by 2 hours; phytase increases mineral availability which may reduce bisphosphonate absorption
Generally no significant drug interactions

Frequently asked questions about Phytase

What is phytase?

Phytase is an enzyme that breaks down phytic acid (phytate), the storage form of phosphorus in seeds, grains, and legumes. Phytate binds minerals, so phytase can free up minerals like iron, zinc, and calcium for absorption.

What is phytase used for?

In supplements and food processing, phytase improves the absorption of minerals from plant foods by breaking down mineral-binding phytate. It is of particular interest for plant-based diets high in grains and legumes.

When should I take phytase?

Take it with high-phytate plant meals (whole grains, legumes, nuts, seeds) so it can act on the phytate as you digest, improving mineral availability. It is often part of a digestive-enzyme blend.

Is phytase safe?

As a digestive enzyme it is generally well tolerated. It simply helps release minerals bound in plant foods. People with enzyme allergies or digestive conditions should check with a doctor.

What is the recommended dosage of Phytase?

The clinically studied dose is Labels commonly list 500 to 1,500 FTU per serving. In absorption studies, 20 to 320 phytase units per 100 g of flour improved iron absorption, with the enzyme mixed into food rather than a capsule. Always follow the product label and check with a healthcare provider for personal advice.

Is Phytase safe, and does it have side effects?

For most healthy adults, Phytase is well tolerated at studied doses. Reported effects can include: Generally well tolerated as a food-grade enzyme Allergic reactions to fungal source in sensitized individuals It may also interact with some medications. Phytase is not right for everyone, so check with a healthcare provider first if you are pregnant or breastfeeding, have a medical condition, or take prescription medication.

Does Phytase interact with any medications?

Possible interactions include: Mineral supplements (iron, zinc, calcium) — synergistic effect; phytase may enhance absorption Bisphosphonates — separate by 2 hours; phytase increases mineral availability which may reduce bisphosphonate absorption If you take prescription medication, check with a pharmacist or doctor before using it.

How strong is the scientific evidence for Phytase?

NutraSmarts rates the evidence for Phytase as Moderate (3 out of 5). It is backed by 2 clinical trials and 8 cited references summarized on this page. A higher rating reflects more, larger, and better-designed human studies.

References(8 citations)

Evidence ratings on NutraSmarts are based on the totality of human clinical research, with emphasis on randomized controlled trials, meta-analyses, and systematic reviews. The references below directly support claims made throughout this page.

  1. Troesch B, Jing H, Laillou A, Fowler A. Absorption studies show that phytase from Aspergillus niger significantly increases iron and zinc bioavailability from phytate-rich foods. Food Nutr Bull. 2013;34(2 Suppl):S90-S101. doi: 10.1177/15648265130342S111.PubMedUsed to support: Human absorption studies demonstrating that supplemental phytase (from Aspergillus niger) significantly improves iron and zinc bioavailability from phytate-rich foods — directly supports core mineral absorption claims.
  2. Gibson RS, Raboy V, King JC. Implications of phytate in plant-based foods for iron and zinc bioavailability, setting dietary requirements, and formulating programs and policies. Nutr Rev. 2018;76(11):793-804. doi: 10.1093/nutrit/nuy028.PubMedUsed to support: Reviews the quantitative impact of phytate on iron and zinc bioavailability in plant-based diets and the strategies (including phytase) to overcome this antinutrient effect; supports claims about critical importance for plant-based and vegetarian populations.
  3. Brnić M, Wegmüller R, Zeder C, Senti G, Hurrell RF. Influence of phytase, EDTA, and polyphenols on zinc absorption in adults from porridges fortified with zinc sulfate or zinc oxide. J Nutr. 2014;144(9):1467-1473. doi: 10.3945/jn.113.185322.PubMedUsed to support: Controlled human intervention study demonstrating phytase significantly enhances zinc absorption from phytate-containing grain porridges; directly supports zinc and mineral bioavailability claims with human data.
  4. Hunt JR, Matthys LA, Johnson LK. Zinc absorption, mineral balance, and blood lipids in women consuming controlled lactoovovegetarian and omnivorous diets for 8 wk. Am J Clin Nutr. 1998;67(3):421-430. doi: 10.1093/ajcn/67.3.421.PubMedUsed to support: Controlled human dietary trial showing reduced zinc absorption on vegetarian diets due to higher phytate intake; context only, since this study tested diets rather than phytase, and it concluded that zinc balance was maintained on both diets.
  5. Lönnerdal B. Dietary factors influencing zinc absorption. J Nutr. 2000;130(5S Suppl):1378S-1383S. doi: 10.1093/jn/130.5.1378S.PubMedUsed to support: Review establishing phytate as the primary dietary inhibitor of zinc absorption and identifying phytase-mediated phytate degradation as the main strategy for improving zinc status; supports mineral absorption benefit claims.
  6. Hurrell RF, Reddy MB, Juillerat MA, et al. Degradation of phytic acid in cereal porridges improves iron absorption by human subjects. Am J Clin Nutr. 2003;77(5):1213-9..PubMedUsed to support: Human iron absorption study in adults. An exogenous phytase was used to fully degrade the phytic acid during manufacture of nine roller-dried cereal foods, and absorption was measured with an extrinsic radioiron label. Dephytinization raised iron absorption from rice porridge from 1.73 percent to 5.34 percent and from wheat porridge from 0.99 percent to 11.54 percent, but the enhancing effect was markedly reduced or removed when the wheat porridge was reconstituted with milk instead of water. This is the study behind the page's first trial card, which previously carried the unresolvable locator naming Egli 2003 and 'Hurrell et al. studies'.
  7. Chondrou T, Adamidi N, Lygouras D, et al. Dietary Phytic Acid, Dephytinization, and Phytase Supplementation Alter Trace Element Bioavailability-A Narrative Review of Human Interventions. Nutrients. 2024;16(23)..PubMedUsed to support: Narrative review of 42 human intervention studies drawn from 3,055 screened articles. Nine of eleven studies of exogenous phytase (82 percent) and eleven of fourteen food dephytinization studies (79 percent) showed increased iron and zinc bioavailability, while thirteen of seventeen studies feeding phytic acid rich foods (77 percent) showed reduced bioavailability. A recent independent synthesis supporting the mineral bioavailability claim, and a record that the human evidence base measures bioavailability rather than long-term mineral status.
  8. Troesch B, van Stuijvenberg ME, Smuts CM, et al. A micronutrient powder with low doses of highly absorbable iron and zinc reduces iron and zinc deficiency and improves weight-for-age Z-scores in South African children. J Nutr. 2011;141(2):237-42..PubMedUsed to support: Randomized, double-blind, controlled trial in 200 schoolchildren with low iron status. A micronutrient powder containing an exogenous phytase active at gut pH, together with NaFeEDTA, ascorbic acid and low doses of iron and zinc, was added to high-phytate maize porridge five days a week for 23 weeks. Serum ferritin and body iron rose and transferrin receptor fell versus control, the prevalence of iron deficiency fell by 30.6 percent and of zinc deficiency by 11.8 percent, and weight-for-age Z-scores improved. This is the only long trial with phytase in the formula to measure mineral status rather than single-meal absorption, and because phytase was combined with other absorption enhancers its individual contribution cannot be separated.